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DNA Thermodynamics & Hybridization Oligo + Target ↔ Duplex





Sequence: # Bases:
Oligo Conc µM
Target Conc µM
Na+, K+ Conc mM
Mg2+ Conc mM
dNTPs Conc mM
5'- -3'


Base Sequence Symbols
Native DNA: A, C, G, T
Inosine: I
Locked nucleic acids: +A, +C, +G, +T