# TOP STRAND, S1 # Oligo ID: R1410 # Sequence (5'to 3'): CCATTGCTACC # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R1411 # Sequence (5'to 3'): GGTAGCAATGG # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: R9 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.010 # [KCl] = 0.0 # [Tris] = 0.002 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 05-05-2004 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- 1.130236081E+001 1.171253190E-002 7.821315098E-003 5.467072150E-003 1.139661702E+001 1.081054125E-002 3.550160177E-003 2.770078750E-003 1.149087759E+001 8.146694200E-003 5.729655069E-003 4.111440800E-003 1.158514251E+001 1.140661175E-002 5.449852063E-003 4.348933250E-003 1.167941178E+001 1.300461600E-002 2.936066750E-003 2.480269000E-003 1.177368542E+001 6.461916025E-003 5.039241965E-003 3.624697962E-003 1.186796341E+001 7.778160000E-003 1.559530684E-003 1.264526500E-003 1.196224575E+001 5.404280750E-003 6.796652524E-003 5.019591750E-003 1.205653246E+001 6.615559150E-003 5.092465093E-003 3.996751850E-003 1.215082352E+001 5.125992750E-003 8.062200062E-003 6.921952250E-003 1.224511893E+001 7.728683500E-003 3.492690212E-003 2.727295500E-003 1.233941870E+001 6.251493750E-003 4.428695064E-003 3.118866750E-003 1.243372283E+001 4.498806500E-003 9.398090638E-003 6.798033000E-003 1.252803131E+001 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