# TOP STRAND, S1 # Oligo ID: R1410 # Sequence (5'to 3'): CCATTGCTACC # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R1411 # Sequence (5'to 3'): GGTAGCAATGG # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: X9 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.0005 # [KCl] = 0.0 # [Tris] = 0.002 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 03-15-2004 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1260000000E+02 .2363555200E-01 .5985981008E-01 .3863544229E-01 .1270000000E+02 .1903591535E-01 .5503182240E-01 .3545268133E-01 .1280000000E+02 .2013474237E-01 .4823131752E-01 .3109355932E-01 .1290000000E+02 .1712413881E-01 .4959162307E-01 .3180353177E-01 .1300000000E+02 .1584397686E-01 .4959890656E-01 .3194594947E-01 .1310000000E+02 .1762982214E-01 .4918277556E-01 .3154650796E-01 .1320000000E+02 .1947935040E-01 .4825600718E-01 .3105547131E-01 .1330000000E+02 .1643560157E-01 .4523813062E-01 .2891351384E-01 .1340000000E+02 .1625286300E-01 .4457437699E-01 .2856083914E-01 .1350000000E+02 .1169236014E-01 .4499893852E-01 .2908963996E-01 .1360000000E+02 .9081489286E-02 .4382695061E-01 .2826580306E-01 .1370000000E+02 .1285542229E-01 .4817097946E-01 .3113070792E-01 .1380000000E+02 .1267591957E-01 .4085328746E-01 .2612876584E-01 .1390000000E+02 .8850339300E-02 .4103416180E-01 .2631124591E-01 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