# TOP STRAND, S1 # Oligo ID: R1443 # Sequence (5'to 3'): GGGACCGCCT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R1444 # Sequence (5'to 3'): AGGCGGTCCC # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: R9 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.010 # [KCl] = 0.0 # [Tris] = 0.002 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 05-06-2004 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- 1.705996165E+001 1.798894200E-002 2.165526538E-002 1.596925400E-002 1.715448360E+001 1.462571450E-002 2.438939392E-002 1.803574275E-002 1.724900990E+001 1.458440325E-002 1.693714290E-002 1.251472337E-002 1.734354056E+001 1.398048625E-002 2.080887942E-002 1.408743688E-002 1.743807557E+001 1.540401800E-002 2.735706930E-002 1.902289100E-002 1.753261494E+001 1.490855150E-002 2.115240153E-002 1.508799425E-002 1.762715867E+001 1.318942000E-002 1.962005914E-002 1.259227500E-002 1.772170676E+001 8.896926250E-003 1.346487351E-002 9.434062250E-003 1.781625920E+001 1.067516850E-002 1.197363632E-002 7.868210750E-003 1.791081599E+001 6.445527250E-003 1.312664940E-002 8.902592750E-003 1.800537715E+001 1.050885400E-002 1.536851479E-002 1.148755100E-002 1.809994266E+001 7.278118425E-003 1.420875529E-002 1.016922579E-002 1.819451252E+001 1.067901525E-002 6.910730095E-003 5.228229750E-003 1.828908675E+001 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