# TOP STRAND, S1 # Oligo ID: R1447 # Sequence (5'to 3'): TTGTAGTCAT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R1448 # Sequence (5'to 3'): ATGACTACAA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: D1 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.125 # [KCl] = 0.0 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 02-11-2004 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- 1.347135576E+001 -4.900801667E-003 7.809402039E-003 5.496934444E-003 1.356571216E+001 2.420689533E-003 2.639221077E-003 1.807183289E-003 1.366007292E+001 -7.126803567E-003 9.103594253E-003 6.665177622E-003 1.375443804E+001 -1.781005000E-003 3.118698281E-003 2.219831333E-003 1.384880751E+001 3.582142000E-003 8.298875055E-003 6.374525333E-003 1.394318134E+001 -9.395010667E-003 9.423103241E-003 7.216826222E-003 1.403755953E+001 -6.606436667E-004 1.101227855E-002 8.244537556E-003 1.413194207E+001 -1.509892333E-003 3.533856159E-003 2.709582222E-003 1.422632897E+001 4.716741700E-003 1.090575877E-002 8.197045533E-003 1.432072023E+001 9.898666667E-004 2.053332554E-003 1.517236444E-003 1.441511584E+001 8.152438000E-003 6.680281249E-003 5.055154667E-003 1.450951581E+001 -6.465202167E-003 5.531839083E-003 3.716871889E-003 1.460392013E+001 6.288592667E-003 8.455816842E-003 5.874058444E-003 1.469832881E+001 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