# TOP STRAND, S1 # Oligo ID: R1447 # Sequence (5'to 3'): TTGTAGTCAT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R1448 # Sequence (5'to 3'): ATGACTACAA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: N1 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.600 # [KCl] = 0.0 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 02-05-2005 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1320000000E+02 .1861360000E-03 .4925548435E-02 .4013084500E-02 .1330000000E+02 -.3249554000E-02 .6771906013E-02 .5268571500E-02 .1340000000E+02 -.6964407500E-02 .1050543405E-01 .7101081250E-02 .1350000000E+02 -.9186681500E-02 .3181143865E-02 .2229945000E-02 .1360000000E+02 -.3048673950E-02 .2733934284E-02 .1924137050E-02 .1370000000E+02 -.5344631750E-03 .1781827829E-02 .1298100912E-02 .1380000000E+02 .1470549575E-02 .2362213464E-02 .2041651425E-02 .1390000000E+02 .1150250000E-04 .8277313726E-02 .6420168000E-02 .1400000000E+02 .6063534200E-02 .1314327218E-01 .9819822900E-02 .1410000000E+02 -.2540651750E-02 .5083127201E-02 .3547669875E-02 .1420000000E+02 -.5012431500E-02 .5013642601E-02 .3779589000E-02 .1430000000E+02 -.6097797650E-02 .8880854638E-02 .6594436175E-02 .1440000000E+02 -.4436683000E-02 .5503498895E-02 .4438692000E-02 .1450000000E+02 -.7254438750E-02 .4321324890E-02 .3091400625E-02 .1460000000E+02 -.1014551550E-01 .4885090338E-02 .4093269500E-02 .1470000000E+02 -.4398925525E-02 .3875835961E-02 .3161251475E-02 .1480000000E+02 -.4527410000E-02 .9096121499E-02 .7527863500E-02 .1490000000E+02 -.6300895000E-02 .8808332154E-02 .6504453000E-02 .1500000000E+02 .3938559100E-02 .3637785896E-02 .2715328900E-02 .1510000000E+02 -.2945985150E-02 .1196537136E-01 .9366804850E-02 .1520000000E+02 .2777552000E-02 .4034690942E-02 .3013526500E-02 .1530000000E+02 .2768700500E-02 .6425320983E-02 .4387728000E-02 .1540000000E+02 -.7918332500E-03 .7634774481E-02 .5426792250E-02 .1550000000E+02 -.1826820250E-02 .1392545566E-01 .9516615250E-02 .1560000000E+02 -.6174056750E-02 .5112730769E-02 .3784723375E-02 .1570000000E+02 -.7022447250E-02 .5458762506E-02 .4027486125E-02 .1580000000E+02 -.3963336750E-03 .2033277296E-02 .1520554663E-02 .1590000000E+02 .4330894875E-02 .5246489905E-02 .4165724125E-02 .1600000000E+02 .5621776000E-02 .1740793415E-02 .1274639500E-02 .1610000000E+02 .7561575850E-02 .5740549784E-02 .4124876225E-02 .1620000000E+02 -.4170681250E-03 .1028732300E-01 .6997295937E-02 .1630000000E+02 .6791292750E-02 .7004530909E-02 .5255313250E-02 .1640000000E+02 .7709695000E-02 .2499314834E-02 .1834112000E-02 .1650000000E+02 .5813172350E-02 .5827138378E-02 .4420301650E-02 .1660000000E+02 .6359241525E-02 .1218548332E-01 .8602924238E-02 .1670000000E+02 .3677802250E-02 .1112646935E-01 .9266227750E-02 .1680000000E+02 .1541787750E-02 .1395196140E-01 .1204864725E-01 .1690000000E+02 .6069736750E-02 .1011529084E-01 .7495372875E-02 .1700000000E+02 .1376217025E-02 .1165980786E-01 .8363616975E-02 .1710000000E+02 .3608533500E-02 .1375544838E-01 .1121061200E-01 .1720000000E+02 .4539379750E-02 .5794613400E-02 .4202968375E-02 .1730000000E+02 .6131985000E-02 .1134863976E-01 .7797192500E-02 .1740000000E+02 .3286435625E-02 .7845458196E-02 .5589742187E-02 .1750000000E+02 .3571397250E-02 .9241179633E-02 .6268769750E-02 .1760000000E+02 -.1632225300E-02 .1330683457E-01 .9624604700E-02 .1770000000E+02 .4014477750E-02 .6824177456E-02 .4783438750E-02 .1780000000E+02 .5877061250E-02 .6272009534E-02 .4438149375E-02 .1790000000E+02 .4243459800E-02 .4095258182E-02 .2927398700E-02 .1800000000E+02 .4636835000E-02 .1688369864E-02 .1175543500E-02 .1810000000E+02 .7362167500E-02 .1346658731E-02 .9424107500E-03 .1820000000E+02 .5685281250E-02 .7171153655E-02 .5472141250E-02 .1830000000E+02 .5813047850E-02 .4904670578E-02 .3657622650E-02 .1840000000E+02 .1034398657E-01 .8330517390E-02 .6846898425E-02 .1850000000E+02 .6690010450E-02 .9283598900E-02 .6752589775E-02 .1860000000E+02 .4262876495E-02 .6290436275E-02 .4899851505E-02 .1870000000E+02 -.6338375100E-02 .7313760017E-02 .5150167450E-02 .1880000000E+02 .5009450000E-04 .9595793031E-02 .7924305500E-02 .1890000000E+02 -.2947850500E-02 .9144830199E-02 .7313114500E-02 .1900000000E+02 -.8376415500E-02 .9390516981E-02 .6801678500E-02 .1910000000E+02 -.5222164400E-02 .5353843415E-02 .3642727800E-02 .1920000000E+02 -.7494125000E-03 .1461749557E-01 .1091565125E-01 .1930000000E+02 -.6947819750E-02 .3724424376E-02 .2767638250E-02 .1940000000E+02 -.1226950199E-01 .9052527241E-02 .6828813015E-02 .1950000000E+02 -.1956009700E-02 .2260119417E-02 .1751265800E-02 .1960000000E+02 -.9877733500E-03 .1609454694E-01 .1059661833E-01 .1970000000E+02 -.8560635825E-02 .1209390067E-01 .8846372088E-02 .1980000000E+02 -.8373971500E-02 .8167249099E-02 .6196928500E-02 .1990000000E+02 -.1328463000E-02 .9772290357E-02 .8305195000E-02 .2000000000E+02 -.4990641750E-02 .9311301888E-02 .6541531875E-02 .2010000000E+02 -.3618812500E-02 .7336350597E-02 .4865949500E-02 .2020000000E+02 -.6117594250E-02 .1118530398E-01 .8705250250E-02 .2030000000E+02 -.3237754500E-02 .1786672541E-01 .1244486275E-01 .2040000000E+02 .1867286225E-02 .3525374222E-02 .2563632388E-02 .2050000000E+02 .7703786650E-03 .7682180584E-02 .5519635835E-02 .2060000000E+02 .4113466250E-02 .1053650338E-01 .7310238125E-02 .2070000000E+02 .4870603925E-02 .5807873660E-02 .5003124075E-02 .2080000000E+02 .4014449250E-02 .6495711497E-02 .4504089125E-02 .2090000000E+02 .6344838250E-02 .6770502981E-02 .5276866750E-02 .2100000000E+02 .5525976500E-02 .1491172666E-01 .1071034825E-01 .2110000000E+02 .8099401000E-02 .8586625389E-02 .6140683000E-02 .2120000000E+02 .5055620250E-02 .1075379833E-01 .7390873125E-02 .2130000000E+02 -.5979894000E-02 .2677789854E-01 .2003381300E-01 .2140000000E+02 .6968257500E-02 .8661023198E-02 .7131972500E-02 .2150000000E+02 .2369167775E-01 .1642692300E-01 .1262816725E-01 .2160000000E+02 .2140269750E-01 .1115469416E-01 .9229232500E-02 .2170000000E+02 .1532799800E-01 .5068033613E-02 .3673442000E-02 .2180000000E+02 .1269426400E-01 .5229120952E-02 .3614651000E-02 .2190000000E+02 .1648158100E-01 .1203291375E-01 .8949214000E-02 .2200000000E+02 .1890996000E-01 .7313709553E-02 .5537910000E-02 .2210000000E+02 .1160031075E-01 .5819152157E-02 .4138744250E-02 .2220000000E+02 .2293601500E-01 .1554002041E-01 .1103011250E-01 .2230000000E+02 .2826938000E-01 .5045246401E-02 .3680365000E-02 .2240000000E+02 .1948296332E-01 .1555799109E-01 .1214352668E-01 .2250000000E+02 .2993583500E-01 .1756035879E-01 .1313280250E-01 .2260000000E+02 .3115439500E-01 .1699115064E-01 .1233717500E-01 .2270000000E+02 .2375531000E-01 .1814892456E-01 .1326986000E-01 .2280000000E+02 .1891384175E-01 .1623996176E-01 .1145158825E-01 .2290000000E+02 .3276081625E-01 .2195179144E-01 .1517355375E-01 .2300000000E+02 .1985567750E-01 .2422768345E-01 .1775560875E-01 .2310000000E+02 .1420782490E-01 .1197511753E-01 .9138260100E-02 .2320000000E+02 .3288040500E-01 .8416078488E-02 .6175882500E-02 .2330000000E+02 .3712167750E-01 .9570405114E-02 .7970697500E-02 .2340000000E+02 .2457960850E-01 .1907262937E-01 .1317862725E-01 .2350000000E+02 .3002181000E-01 .1564775873E-01 .1340872500E-01 .2360000000E+02 .2925075000E-01 .9582800428E-02 .6306280000E-02 .2370000000E+02 .2384978000E-01 .2322325920E-01 .1741422500E-01 .2380000000E+02 .2941932575E-01 .1524999334E-01 .1097350637E-01 .2390000000E+02 .2260278000E-01 .2710578131E-01 .2011672000E-01 .2400000000E+02 .3309041000E-01 .1871845293E-01 .1508918000E-01 .2410000000E+02 .2922430900E-01 .2381634302E-01 .1766539650E-01 .2420000000E+02 .2642589600E-01 .1398563013E-01 .1054611900E-01 .2430000000E+02 .3508335000E-01 .1827080908E-01 .1366597000E-01 .2440000000E+02 .3589621825E-01 .1896322889E-01 .1421194762E-01 .2450000000E+02 .3004100975E-01 .1662592403E-01 .1226454038E-01 .2460000000E+02 .3623534500E-01 .1268682517E-01 .9235087500E-02 .2470000000E+02 .3102421225E-01 .1747751651E-01 .1434866775E-01 .2480000000E+02 .3712180975E-01 .1967133404E-01 .1404168025E-01 .2490000000E+02 .3640548750E-01 .2000237481E-01 .1643593750E-01 .2500000000E+02 .3903607500E-01 .2041027569E-01 .1646987500E-01 .2510000000E+02 .4562211750E-01 .1914067672E-01 .1393466375E-01 .2520000000E+02 .3967311850E-01 .2408872452E-01 .1734980150E-01 .2530000000E+02 .4489181500E-01 .2189957915E-01 .1749651000E-01 .2540000000E+02 .5017095361E-01 .3369393426E-01 .2513372959E-01 .2550000000E+02 .4679383000E-01 .1510388711E-01 .1011091000E-01 .2560000000E+02 .5294478250E-01 .2390136387E-01 .1703526750E-01 .2570000000E+02 .5296645500E-01 .2587653572E-01 .1810493000E-01 .2580000000E+02 .5312273500E-01 .2627333910E-01 .1809859250E-01 .2590000000E+02 .5561185750E-01 .2525735721E-01 .1797224375E-01 .2600000000E+02 .5713843750E-01 .2690906232E-01 .1914901375E-01 .2610000000E+02 .6125647500E-01 .2271235571E-01 .1771251500E-01 .2620000000E+02 .6839347500E-01 .1788988658E-01 .1383458500E-01 .2630000000E+02 .6807637000E-01 .2928571893E-01 .2433269000E-01 .2640000000E+02 .7077702000E-01 .2807873021E-01 .2324073000E-01 .2650000000E+02 .7060930750E-01 .2117196604E-01 .1743246750E-01 .2660000000E+02 .6442501500E-01 .3295905206E-01 .2682360000E-01 .2670000000E+02 .7510189750E-01 .2967362720E-01 .2186867750E-01 .2680000000E+02 .7734307250E-01 .3519964395E-01 .2610406750E-01 .2690000000E+02 .7390621000E-01 .3004750669E-01 .2179255500E-01 .2700000000E+02 .7007629250E-01 .4257319865E-01 .3240635750E-01 .2710000000E+02 .8145677500E-01 .2902478306E-01 .2092459750E-01 .2720000000E+02 .7860987750E-01 .3529142501E-01 .2931592250E-01 .2730000000E+02 .9489468750E-01 .3724818710E-01 .2901881250E-01 .2740000000E+02 .8421920750E-01 .4356376453E-01 .3183774250E-01 .2750000000E+02 .9146150750E-01 .3636072030E-01 .2872204250E-01 .2760000000E+02 .1005113450E-00 .4827622811E-01 .3421853250E-01 .2770000000E+02 .9615124500E-01 .3620772603E-01 .2786750500E-01 .2780000000E+02 .1014137300E-00 .2949362131E-01 .2031017000E-01 .2790000000E+02 .1004537050E-00 .3777571182E-01 .2997189500E-01 .2800000000E+02 .9645928000E-01 .4144310960E-01 .3408597000E-01 .2810000000E+02 .9324308500E-01 .4898523252E-01 .3872286500E-01 .2820000000E+02 .1057065850E-00 .4195968910E-01 .3132306500E-01 .2830000000E+02 .1084949500E-00 .4165752373E-01 .3062330000E-01 .2840000000E+02 .1232970175E-00 .2792149767E-01 .2252293250E-01 .2850000000E+02 .1242227025E-00 .5459743798E-01 .4416479750E-01 .2860000000E+02 .1231203900E-00 .5018401380E-01 .4039756000E-01 .2870000000E+02 .1224710250E-00 .4250341115E-01 .3422947500E-01 .2880000000E+02 .1288745875E-00 .3982433054E-01 .3064976250E-01 .2890000000E+02 .1358158875E-00 .4134284925E-01 .3456726250E-01 .2900000000E+02 .1359716600E-00 .5320263941E-01 .4296104000E-01 .2910000000E+02 .1329698175E-00 .4232259174E-01 .3356788250E-01 .2920000000E+02 .1376406750E-00 .4590081372E-01 .3729722500E-01 .2930000000E+02 .1451602125E-00 .5655874528E-01 .4300393750E-01 .2940000000E+02 .1472295475E-00 .5522154274E-01 .4325660250E-01 .2950000000E+02 .1491212950E-00 .5528968153E-01 .4369105500E-01 .2960000000E+02 .1519010050E-00 .5036552384E-01 .3927234500E-01 .2970000000E+02 .1616316150E-00 .4729574998E-01 .3696588500E-01 .2980000000E+02 .1563684150E-00 .5718256197E-01 .4521638500E-01 .2990000000E+02 .1655316000E-00 .6666862161E-01 .5415885000E-01 .3000000000E+02 .1783713250E-00 .5604725946E-01 .4498532500E-01 .3010000000E+02 .1767917750E-00 .5705324385E-01 .4553672500E-01 .3020000000E+02 .1897770000E-00 .4825332501E-01 .4039735000E-01 .3030000000E+02 .2001333250E-00 .4570016293E-01 .3885152500E-01 .3040000000E+02 .1959390750E-00 .6100514615E-01 .4924417500E-01 .3050000000E+02 .1933825250E-00 .6409718160E-01 .5225077500E-01 .3060000000E+02 .2028626000E-00 .5408288920E-01 .4442350000E-01 .3070000000E+02 .2026772750E-00 .6129189683E-01 .4778432500E-01 .3080000000E+02 .2129961000E-00 .6080373931E-01 .4776480000E-01 .3090000000E+02 .2089553000E-00 .5178843864E-01 .4045115000E-01 .3100000000E+02 .2191750750E-00 .6201349121E-01 .5119507500E-01 .3110000000E+02 .2289217500E-00 .5555097043E-01 .4682935000E-01 .3120000000E+02 .2259142000E-00 .5812070313E-01 .4697865000E-01 .3130000000E+02 .2349326750E-00 .5997686869E-01 .4718007500E-01 .3140000000E+02 .2374868750E-00 .5923402558E-01 .4308617500E-01 .3150000000E+02 .2506091250E-00 .5981610263E-01 .4738937500E-01 .3160000000E+02 .2522412250E-00 .6950812580E-01 .5407972500E-01 .3170000000E+02 .2581726000E-00 .5986549166E-01 .4729745000E-01 .3180000000E+02 .2633168000E-00 .5386465528E-01 .4375245000E-01 .3190000000E+02 .2690297000E-00 .4653267466E-01 .3837065000E-01 .3200000000E+02 .2829425250E-00 .4298188910E-01 .3508077500E-01 .3210000000E+02 .2895465250E-00 .4799902898E-01 .3609467500E-01 .3220000000E+02 .2819194500E-00 .6647356940E-01 .5060845000E-01 .3230000000E+02 .2916152750E-00 .5889783221E-01 .4533842500E-01 .3240000000E+02 .2969095750E-00 .4936748653E-01 .3506773750E-01 .3250000000E+02 .3144737250E-00 .5393070081E-01 .3895771250E-01 .3260000000E+02 .3130064250E-00 .5159821971E-01 .3809797500E-01 .3270000000E+02 .3130479000E-00 .5590030125E-01 .4192530000E-01 .3280000000E+02 .3127910000E-00 .5822853894E-01 .4677065000E-01 .3290000000E+02 .3384691250E-00 .6125798255E-01 .4747052500E-01 .3300000000E+02 .3330512750E-00 .5316638004E-01 .3920642500E-01 .3310000000E+02 .3355779000E-00 .5317557703E-01 .4131805000E-01 .3320000000E+02 .3409235250E-00 .5291864812E-01 .4219347500E-01 .3330000000E+02 .3500612250E-00 .5605501007E-01 .4359507500E-01 .3340000000E+02 .3592004500E-00 .5940654565E-01 .4483125000E-01 .3350000000E+02 .3738442500E-00 .5236109824E-01 .3760250000E-01 .3360000000E+02 .3796928750E-00 .5491793715E-01 .3946537500E-01 .3370000000E+02 .3812027750E-00 .6049033384E-01 .4673627500E-01 .3380000000E+02 .3886783500E-00 .4463727402E-01 .3655915000E-01 .3390000000E+02 .3958221750E-00 .4486347529E-01 .3469202500E-01 .3400000000E+02 .4037830250E-00 .4868339828E-01 .3446567500E-01 .3410000000E+02 .4073669500E-00 .4752812423E-01 .3685920000E-01 .3420000000E+02 .4172785000E-00 .5073383671E-01 .4158250000E-01 .3430000000E+02 .4190892250E-00 .4458266439E-01 .3471582500E-01 .3440000000E+02 .4271152750E-00 .4341107109E-01 .3480567500E-01 .3450000000E+02 .4312176250E-00 .4527822559E-01 .3724667500E-01 .3460000000E+02 .4383340750E-00 .5238033395E-01 .4374482500E-01 .3470000000E+02 .4472868000E-00 .5921765497E-01 .4566895000E-01 .3480000000E+02 .4522452000E-00 .6764338682E-01 .4775500000E-01 .3490000000E+02 .4728486000E-00 .3988780128E-01 .3057380000E-01 .3500000000E+02 .4657737750E-00 .5327364975E-01 .4139077500E-01 .3510000000E+02 .4711090750E-00 .5087658081E-01 .4046227500E-01 .3520000000E+02 .4841320250E-00 .4064267615E-01 .3212902500E-01 .3530000000E+02 .4842590500E-00 .6251896122E-01 .4565785000E-01 .3540000000E+02 .5028311500E-00 .4757922272E-01 .3613920000E-01 .3550000000E+02 .5141098250E-00 .4419440590E-01 .3635637500E-01 .3560000000E+02 .5219113000E-00 .4957148231E-01 .4149860000E-01 .3570000000E+02 .5234034000E-00 .5133024846E-01 .4282960000E-01 .3580000000E+02 .5364505750E-00 .4457088654E-01 .3494487500E-01 .3590000000E+02 .5286626000E-00 .5540388882E-01 .4401775000E-01 .3600000000E+02 .5435640750E-00 .5318550151E-01 .4060847500E-01 .3610000000E+02 .5418491750E-00 .5903132930E-01 .4898017500E-01 .3620000000E+02 .5567215750E-00 .5867332690E-01 .4849382500E-01 .3630000000E+02 .5656152500E-00 .5499984117E-01 .4270605000E-01 .3640000000E+02 .5659659250E-00 .5649018339E-01 .4719337500E-01 .3650000000E+02 .5795471250E-00 .5152102420E-01 .3937592500E-01 .3660000000E+02 .5814088000E-00 .5500624323E-01 .4458055000E-01 .3670000000E+02 .5974468000E-00 .3974047985E-01 .3207820000E-01 .3680000000E+02 .5997378500E-00 .4264504256E-01 .3356040000E-01 .3690000000E+02 .6062616500E-00 .4789634579E-01 .3744270000E-01 .3700000000E+02 .6129715000E-00 .5223815721E-01 .4146025000E-01 .3710000000E+02 .6183421750E-00 .5997730187E-01 .4981182500E-01 .3720000000E+02 .6197567500E-00 .6204885631E-01 .5126760000E-01 .3730000000E+02 .6244234750E-00 .5834034766E-01 .4893962500E-01 .3740000000E+02 .6256238750E-00 .5720030881E-01 .4818712500E-01 .3750000000E+02 .6336354500E-00 .6163297136E-01 .5177795000E-01 .3760000000E+02 .6395500250E-00 .6209439680E-01 .5142262500E-01 .3770000000E+02 .6545242250E-00 .6263846100E-01 .5262062500E-01 .3780000000E+02 .6574873750E-00 .5116649277E-01 .4411742500E-01 .3790000000E+02 .6652619250E-00 .5732205349E-01 .4255867500E-01 .3800000000E+02 .6706273250E-00 .6024892197E-01 .4896147500E-01 .3810000000E+02 .6744751500E-00 .5928903313E-01 .4805590000E-01 .3820000000E+02 .6817611500E-00 .6379553017E-01 .5233400000E-01 .3830000000E+02 .6800779250E-00 .5611423893E-01 .4682287500E-01 .3840000000E+02 .6931488000E-00 .6795569847E-01 .5441595000E-01 .3850000000E+02 .7084530000E-00 .7559872588E-01 .6115300000E-01 .3860000000E+02 .7133664000E-00 .5445010736E-01 .4431020000E-01 .3870000000E+02 .7186020500E-00 .5109830103E-01 .4225720000E-01 .3880000000E+02 .7304948750E-00 .5710741903E-01 .4645537500E-01 .3890000000E+02 .7341686250E-00 .5716554289E-01 .4739872500E-01 .3900000000E+02 .7315745500E-00 .5227811444E-01 .4202350000E-01 .3910000000E+02 .7385795500E-00 .5105334980E-01 .4172640000E-01 .3920000000E+02 .7470198750E-00 .4851701606E-01 .4013062500E-01 .3930000000E+02 .7496624250E-00 .5862687949E-01 .4787322500E-01 .3940000000E+02 .7491742500E-00 .6812269143E-01 .5617290000E-01 .3950000000E+02 .7554656000E-00 .5550097791E-01 .4252085000E-01 .3960000000E+02 .7697605250E-00 .5069980852E-01 .4167657500E-01 .3970000000E+02 .7770655250E-00 .4513759415E-01 .3796037500E-01 .3980000000E+02 .7777924250E-00 .5652060193E-01 .4446562500E-01 .3990000000E+02 .7761031250E-00 .5501629821E-01 .4691407500E-01 .4000000000E+02 .7770477250E-00 .5160626143E-01 .4093302500E-01 .4010000000E+02 .7901840250E-00 .4858488738E-01 .4001347500E-01 .4020000000E+02 .7999265250E-00 .5311484076E-01 .4404512500E-01 .4030000000E+02 .8076040500E-00 .5447623376E-01 .4547050000E-01 .4040000000E+02 .8113926250E-00 .5659421955E-01 .4629432500E-01 .4050000000E+02 .8183902500E-00 .5555395387E-01 .4446700000E-01 .4060000000E+02 .8212554000E-00 .5055456286E-01 .4232980000E-01 .4070000000E+02 .8272431250E-00 .5103304997E-01 .4258667500E-01 .4080000000E+02 .8252315000E-00 .5511503950E-01 .4561285000E-01 .4090000000E+02 .8227564000E-00 .5792001584E-01 .4724230000E-01 .4100000000E+02 .8358825750E-00 .4704000246E-01 .3924002500E-01 .4110000000E+02 .8454268750E-00 .5452697870E-01 .4211782500E-01 .4120000000E+02 .8494638000E-00 .4444407317E-01 .3651355000E-01 .4130000000E+02 .8447434500E-00 .6369375300E-01 .4921065000E-01 .4140000000E+02 .8528617500E-00 .3484880500E-01 .2798110000E-01 .4150000000E+02 .8590175000E-00 .4931622227E-01 .3829800000E-01 .4160000000E+02 .8614095000E-00 .4145191318E-01 .3226175000E-01 .4170000000E+02 .8658337750E-00 .4462125986E-01 .3226407500E-01 .4180000000E+02 .8680346250E-00 .3917149970E-01 .3155797500E-01 .4190000000E+02 .8755721750E-00 .4205620694E-01 .3482732500E-01 .4200000000E+02 .8853750000E-00 .5335437828E-01 .4306290000E-01 .4210000000E+02 .8740978500E-00 .4336342211E-01 .3190350000E-01 .4220000000E+02 .8708104000E-00 .3802975990E-01 .2724210000E-01 .4230000000E+02 .8821920000E-00 .4211988049E-01 .3536025000E-01 .4240000000E+02 .8852208500E-00 .4340970640E-01 .3456090000E-01 .4250000000E+02 .8888956000E-00 .5226287406E-01 .4408750000E-01 .4260000000E+02 .8947381000E-00 .4058436964E-01 .3247000000E-01 .4270000000E+02 .8969556250E-00 .4054171935E-01 .3331382500E-01 .4280000000E+02 .9116955250E-00 .3104784322E-01 .2557337500E-01 .4290000000E+02 .9046015750E-00 .4871001365E-01 .3867322500E-01 .4300000000E+02 .9096927750E-00 .4728435690E-01 .3878182500E-01 .4310000000E+02 .9062992500E-00 .4743697572E-01 .3703600000E-01 .4320000000E+02 .9113291250E-00 .3611359071E-01 .2647877500E-01 .4330000000E+02 .9058420750E-00 .3610979439E-01 .2666337500E-01 .4340000000E+02 .9229832000E-00 .4516629085E-01 .3428730000E-01 .4350000000E+02 .9228764750E-00 .4231200424E-01 .3184032500E-01 .4360000000E+02 .9251455250E-00 .3915825669E-01 .2796112500E-01 .4370000000E+02 .9233048750E-00 .3034602836E-01 .2456387500E-01 .4380000000E+02 .9204788000E-00 .3122440196E-01 .2450380000E-01 .4390000000E+02 .9240815750E-00 .3815023345E-01 .3173862500E-01 .4400000000E+02 .9303971750E-00 .4397907515E-01 .3665872500E-01 .4410000000E+02 .9278300500E-00 .4385003195E-01 .3747660000E-01 .4420000000E+02 .9318745250E-00 .4812894267E-01 .3384252500E-01 .4430000000E+02 .9345155500E-00 .3642349878E-01 .2916385000E-01 .4440000000E+02 .9458171500E-00 .5689526644E-01 .4600105000E-01 .4450000000E+02 .9379919750E-00 .3627900155E-01 .2804992500E-01 .4460000000E+02 .9471244000E-00 .3382451957E-01 .2779125000E-01 .4470000000E+02 .9450361000E-00 .3560587766E-01 .2476430000E-01 .4480000000E+02 .9455599500E-00 .3734071992E-01 .2844365000E-01 .4490000000E+02 .9466983000E-00 .4384605553E-01 .3483220000E-01 .4500000000E+02 .9445314750E-00 .4636445727E-01 .3725872500E-01 .4510000000E+02 .9509458250E-00 .3658976465E-01 .2867797500E-01 .4520000000E+02 .9591160500E-00 .4848171237E-01 .4035585000E-01 .4530000000E+02 .9537969750E-00 .3461020619E-01 .2922207500E-01 .4540000000E+02 .9552797000E-00 .3699827735E-01 .2859630000E-01 .4550000000E+02 .9554374500E-00 .3021110195E-01 .2359195000E-01 .4560000000E+02 .9505044500E-00 .2138927220E-01 .1669640000E-01 .4570000000E+02 .9569339000E-00 .2189311142E-01 .1774050000E-01 .4580000000E+02 .9589902750E-00 .2975596448E-01 .2238487500E-01 .4590000000E+02 .9606932000E-00 .2956834735E-01 .2487645000E-01 .4600000000E+02 .9676791250E-00 .3147067353E-01 .2590887500E-01 .4610000000E+02 .9618112250E-00 .3468998508E-01 .2958257500E-01 .4620000000E+02 .9732318000E-00 .2557688498E-01 .1801565000E-01 .4630000000E+02 .9654725500E-00 .2766394648E-01 .2343205000E-01 .4640000000E+02 .9775831250E-00 .1433423869E-01 .1175757500E-01 .4650000000E+02 .9767694500E-00 .1513725333E-01 .1249660000E-01 .4660000000E+02 .9723208750E-00 .1884103661E-01 .1433837500E-01 .4670000000E+02 .9696277000E-00 .1804933924E-01 .1304840000E-01 .4680000000E+02 .9716467750E-00 .1379055545E-01 .9860475000E-02 .4690000000E+02 .9730373500E-00 .7112761130E-02 .5939100000E-02 .4700000000E+02 .9757570000E-00 .1648574148E-01 .1157700000E-01 .4710000000E+02 .9909264500E-00 .1835437327E-01 .1372747500E-01 .4720000000E+02 .1000966275E+01 .1147493905E-01 .7882362500E-02 .4730000000E+02 .9902669750E-00 .9665627144E-02 .7026337500E-02 .4740000000E+02 .9934462000E-00 .2686092983E-01 .1969650000E-01 .4750000000E+02 .9889498250E-00 .2028164421E-01 .1454351250E-01 .4760000000E+02 .9837517250E-00 .1441058132E-01 .1071656250E-01 .4770000000E+02 .9927237500E-00 .2934465736E-01 .1890362500E-01 .4780000000E+02 .9884496500E-00 .2026695413E-01 .1504275000E-01 .4790000000E+02 .9924876250E-00 .2065313964E-01 .1682087500E-01 .4800000000E+02 .9909166750E-00 .1682492512E-01 .1222327500E-01 .4810000000E+02 .9954291000E-00 .1360780507E-01 .9912850000E-02 .4820000000E+02 .9834419500E-00 .1309543176E-01 .9432350000E-02 .4830000000E+02 .9914963250E-00 .1490642529E-01 .1109611250E-01 .4840000000E+02 .9897055000E-00 .1202197334E-01 .9443800000E-02 .4850000000E+02 .9888031250E-00 .9177030681E-02 .6833675000E-02 .4860000000E+02 .9910529750E-00 .5645153988E-02 .4193125000E-02 .4870000000E+02 .9982932750E-00 .2325561874E-01 .1507327500E-01 .4880000000E+02 .9971369750E-00 .1026318571E-01 .8169525000E-02 .4890000000E+02 .9917371000E-00 .9069500257E-02 .6599950000E-02 .4900000000E+02 .9976120250E-00 .5534072992E-02 .4788475000E-02 .4910000000E+02 .9965539000E-00 .1379178279E-01 .1119460000E-01 .4920000000E+02 .9996019500E-00 .1236803991E-01 .9040550000E-02 .4930000000E+02 .1004015350E+01 .1150943965E-01 .8574825000E-02 .4940000000E+02 .1009218000E+01 .1150961520E-01 .8473000000E-02 .4950000000E+02 .9922146750E-00 .1815758435E-01 .1297457500E-01 .4960000000E+02 .9990470750E-00 .1545618890E-01 .1071146250E-01 .4970000000E+02 .1001825775E+01 .1018587794E-01 .7400112500E-02 .4980000000E+02 .9987253250E-00 .8229936339E-02 .5943675000E-02 .4990000000E+02 .9977211500E-00 .7705198040E-02 .5811850000E-02 .5000000000E+02 .9961601500E-00 .8173913028E-02 .5964850000E-02 .5010000000E+02 .9990777500E-00 .1061325968E-01 .7562750000E-02 .5020000000E+02 .9950539000E-00 .9952550853E-02 .7328200000E-02 .5030000000E+02 .1003504825E+01 .5914116928E-02 .5068675000E-02 .5040000000E+02 .9967904250E-00 .2433429009E-01 .1872857500E-01 .5050000000E+02 .1002326375E+01 .1567477210E-01 .1321762500E-01 .5060000000E+02 .1007695250E+01 .6035694126E-02 .4330250000E-02 .5070000000E+02 .1003104200E+01 .1072166717E-01 .7589700000E-02 .5080000000E+02 .1007079300E+01 .7799853526E-02 .5776700000E-02 .5090000000E+02 .1003157600E+01 .8478862507E-02 .7149900000E-02 .5100000000E+02 .1008100275E+01 .2110383161E-01 .1548158750E-01 .5110000000E+02 .1004635675E+01 .1365252749E-01 .1007798750E-01 .5120000000E+02 .1006591800E+01 .1030850934E-01 .7191200000E-02 .5130000000E+02 .9974050750E-00 .5744748394E-02 .4740425000E-02 .5140000000E+02 .1004651225E+01 .1452519439E-01 .1231477500E-01 .5150000000E+02 .1005808425E+01 .1041395281E-01 .7990075000E-02 .5160000000E+02 .1003270225E+01 .1463339130E-01 .9344662500E-02 .5170000000E+02 .1001129450E+01 .8081133621E-02 .6532050000E-02 .5180000000E+02 .9979721250E-00 .7504419176E-02 .5909875000E-02 .5190000000E+02 .1003156925E+01 .4114500134E-02 .3087075000E-02 .5200000000E+02 .1000478425E+01 .6026477161E-02 .4345575000E-02 .5210000000E+02 .1005569750E+01 .4058883457E-02 .3108750000E-02 .5220000000E+02 .1005649300E+01 .5671067104E-02 .4118050000E-02 .5230000000E+02 .1006407325E+01 .7663373828E-02 .5309512500E-02 .5240000000E+02 .1006383700E+01 .8511366735E-02 .5627950000E-02 .5250000000E+02 .1007924350E+01 .1159202347E-01 .8465150000E-02 .5260000000E+02 .1012514750E+01 .1034633469E-01 .7412125000E-02 .5270000000E+02 .1007991525E+01 .1267922178E-01 .1021497500E-01 .5280000000E+02 .1006692625E+01 .1235070120E-01 .8717375000E-02 .5290000000E+02 .1004292250E+01 .3616295653E-02 .3115250000E-02 .5300000000E+02 .1002572350E+01 .1275156658E-01 .8982150000E-02 .5310000000E+02 .1005611000E+01 .4022401687E-02 .3205500000E-02 .5320000000E+02 .1007753950E+01 .1864516852E-01 .1251055000E-01 .5330000000E+02 .1010913150E+01 .1499135788E-01 .1269885000E-01 .5340000000E+02 .1010128000E+01 .4233413753E-02 .2924000000E-02 .5350000000E+02 .1007735500E+01 .6869180956E-02 .4955250000E-02 .5360000000E+02 .1008544275E+01 .1275917002E-01 .9479725000E-02 .5370000000E+02 .1000534025E+01 .1064077012E-01 .7387975000E-02 .5380000000E+02 .1006553025E+01 .1075259239E-01 .8398475000E-02 .5390000000E+02 .1006652625E+01 .8624998293E-02 .6378375000E-02 .5400000000E+02 .1008366850E+01 .1585543014E-01 .1313815000E-01 .5410000000E+02 .1004175775E+01 .6475140987E-02 .4807225000E-02 .5420000000E+02 .9965404750E-00 .7397320721E-02 .5679025000E-02 .5430000000E+02 .9989221500E-00 .2872961184E-02 .1936150000E-02 .5440000000E+02 .1001611475E+01 .2623622401E-02 .1843025000E-02 .5450000000E+02 .9981103000E-00 .1617654896E-01 .1231370000E-01 .5460000000E+02 .9862632500E-00 .1348780695E-01 .1124515000E-01 .5470000000E+02 .9963592500E-00 .3466507516E-02 .2421375000E-02 .5480000000E+02 .1006129500E+01 .5538606594E-02 .4642000000E-02 .5490000000E+02 .1006103350E+01 .5965110166E-02 .4741150000E-02 .5500000000E+02 .9922042500E-00 .5663209960E-02 .4209175000E-02 .5510000000E+02 .1000640925E+01 .1479715548E-01 .9672075000E-02 .5520000000E+02 .1001086300E+01 .6933161110E-02 .5967700000E-02 .5530000000E+02 .1001239775E+01 .5211824690E-02 .4484725000E-02 .5540000000E+02 .9980223250E-00 .3233557735E-02 .2258275000E-02 .5550000000E+02 .1000694600E+01 .1166974119E-01 .8601600000E-02 .5560000000E+02 .1001388800E+01 .2895497453E-01 .2020620000E-01 .5570000000E+02 .9891368250E-00 .1313257703E-01 .9250275000E-02 .5580000000E+02 .9996575250E-00 .1428088947E-01 .1061621250E-01 .5590000000E+02 .1006132500E+01 .5074965911E-02 .3760750000E-02 .5600000000E+02 .1022504750E+01 .1125761755E-01 .8212250000E-02 .5610000000E+02 .1007400250E+01 .4690814952E-02 .3618250000E-02 .5620000000E+02 .1000715200E+01 .6698968599E-02 .4600800000E-02 .5630000000E+02 .1001429375E+01 .8265025926E-02 .5940125000E-02 .5640000000E+02 .1000398300E+01 .1723755157E-01 .1423120000E-01 .5650000000E+02 .9929055250E-00 .1082268811E-01 .6944425000E-02 .5660000000E+02 .9936743750E-00 .1482884015E-01 .1035073750E-01 .5670000000E+02 .9966963250E-00 .1019918656E-01 .7204662500E-02 .5680000000E+02 .9973854000E-00 .1267227041E-01 .9147750000E-02 .5690000000E+02 .9982712500E-00 .8240553802E-02 .6116550000E-02 .5700000000E+02 .1001198850E+01 .4886869704E-02 .4156150000E-02 .5710000000E+02 .1002210975E+01 .1155284084E-01 .8166037500E-02 .5720000000E+02 .1003324250E+01 .1516793980E-01 .1081037500E-01 .5730000000E+02 .1001159425E+01 .9966960144E-02 .7199575000E-02 .5740000000E+02 .1008926725E+01 .9671718129E-02 .6139912500E-02 .5750000000E+02 .1004039275E+01 .7572324357E-02 .5630587500E-02 .5760000000E+02 .9996649750E-00 .7493564586E-02 .5818025000E-02 .5770000000E+02 .9909931000E-00 .4725443193E-02 .3928250000E-02 .5780000000E+02 .9857752500E-00 .8477516616E-02 .6224725000E-02 .5790000000E+02 .9903744500E-00 .1038305222E-01 .7501700000E-02 .5800000000E+02 .9927444750E-00 .1370111311E-01 .1020023750E-01 .5810000000E+02 .9889677750E-00 .4720345642E-02 .3779825000E-02 .5820000000E+02 .1002070150E+01 .2144521443E-01 .1614035000E-01 .5830000000E+02 .1003986450E+01 .6698186685E-02 .5283550000E-02 .5840000000E+02 .9997270500E-00 .9230851093E-02 .6691950000E-02 .5850000000E+02 .1003708775E+01 .1045015961E-01 .7801612500E-02 .5860000000E+02 .9898309250E-00 .1257787173E-01 .9841075000E-02 .5870000000E+02 .9927285000E-00 .3973294757E-02 .3361800000E-02 .5880000000E+02 .1000616725E+01 .7336140468E-02 .5732275000E-02 .5890000000E+02 .1000114050E+01 .7292507750E-02 .4969475000E-02 .5900000000E+02 .1001181450E+01 .1264592612E-01 .1034055000E-01