# TOP STRAND, S1 # Oligo ID: R1447 # Sequence (5'to 3'): TTGTAGTCAT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R1448 # Sequence (5'to 3'): ATGACTACAA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: Q9 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.0015 # [KCl] = 0.0 # [Tris] = 0.002 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 03-30-2004 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- 1.233941870E+001 1.787491625E-003 1.477494209E-002 1.096153587E-002 1.243372283E+001 -9.547500000E-004 1.424227028E-002 9.910139500E-003 1.252803131E+001 -3.108347500E-004 1.930085378E-002 1.638234731E-002 1.262234416E+001 -3.139694250E-003 1.802455773E-002 1.374762600E-002 1.271666135E+001 1.349970000E-003 9.966642460E-003 8.012923750E-003 1.281098291E+001 -3.251366363E-004 1.315656326E-002 1.001443302E-002 1.290530882E+001 2.680449700E-003 1.408633322E-002 1.068215515E-002 1.299963908E+001 -8.713383750E-004 1.202055081E-002 9.909260281E-003 1.309397370E+001 7.545967250E-003 1.001172104E-002 8.161180250E-003 1.318831268E+001 5.615576250E-004 1.516821732E-002 1.150733703E-002 1.328265602E+001 9.875655000E-004 9.065356075E-003 7.198638875E-003 1.337700371E+001 3.323008750E-003 1.339850083E-002 1.129293844E-002 1.347135576E+001 1.443707787E-003 8.196573333E-003 6.383728213E-003 1.356571216E+001 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5.363154463E-003 1.498158099E+001 9.409170000E-004 6.789687158E-003 4.994333750E-003 1.507600710E+001 -6.748855000E-004 1.165856080E-002 7.975142375E-003 1.517043756E+001 -1.029506212E-003 8.190104279E-003 5.756950037E-003 1.526487238E+001 1.621679375E-003 1.568715930E-002 1.277208237E-002 1.535931156E+001 9.229092750E-004 7.819142791E-003 5.541458294E-003 1.545375509E+001 3.909519300E-003 1.355479971E-002 1.070765853E-002 1.554820298E+001 6.779722724E-003 1.182396076E-002 9.174901138E-003 1.564265522E+001 -7.924854500E-004 9.752279360E-003 7.171472800E-003 1.573711182E+001 5.919686750E-003 1.342019869E-002 1.154302244E-002 1.583157278E+001 -2.224691911E-003 7.970495142E-003 6.314818339E-003 1.592603809E+001 6.121892500E-004 1.185434843E-002 9.047264750E-003 1.602050776E+001 3.079345250E-003 6.202894211E-003 5.321974312E-003 1.611498179E+001 6.766471875E-003 1.589583287E-002 1.292183738E-002 1.620946017E+001 8.492856000E-003 1.391774840E-002 1.216220500E-002 1.630394291E+001 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