# TOP STRAND, S1 # Oligo ID: R1447 # Sequence (5'to 3'): TTGTAGTCAT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R1448 # Sequence (5'to 3'): ATGACTACAA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: R9 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.010 # [KCl] = 0.0 # [Tris] = 0.002 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 04-28-2004 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- 1.045425098E+001 1.472130750E-002 2.801606555E-003 1.941822500E-003 1.054846798E+001 1.319963250E-002 5.310771836E-003 4.246362500E-003 1.064268934E+001 1.236418025E-002 4.515260516E-003 2.867364750E-003 1.073691505E+001 8.894193250E-003 2.652445008E-003 2.053779250E-003 1.083114512E+001 1.119049225E-002 5.051677437E-003 4.262917750E-003 1.092537955E+001 1.211126475E-002 4.249506748E-003 2.991240250E-003 1.101961833E+001 1.600867125E-002 8.672317191E-003 7.098088750E-003 1.111386147E+001 1.115330500E-002 1.748747422E-003 1.176015000E-003 1.120810896E+001 1.295271475E-002 4.970224608E-003 3.990800250E-003 1.130236081E+001 8.588974250E-003 2.794739563E-003 2.074329625E-003 1.139661702E+001 1.003565225E-002 2.733986756E-003 1.818551625E-003 1.149087759E+001 8.728275750E-003 1.911710395E-003 1.385855875E-003 1.158514251E+001 6.665057050E-003 4.953996077E-003 3.644088925E-003 1.167941178E+001 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