# TOP STRAND, S1 # Oligo ID: R1447 # Sequence (5'to 3'): TTGTAGTCAT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R1448 # Sequence (5'to 3'): ATGACTACAA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: V1 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.050 # [KCl] = 0.0 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 02-22-2004 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- 1.271666135E+001 -5.747457500E-003 1.155897364E-002 1.025389750E-002 1.281098291E+001 -9.230362750E-003 7.770000415E-003 6.259538312E-003 1.290530882E+001 -6.082696500E-003 7.536937790E-003 4.878845500E-003 1.299963908E+001 -1.020561463E-002 6.640841674E-003 5.360814219E-003 1.309397370E+001 -9.189919750E-003 8.956700652E-003 6.440087688E-003 1.318831268E+001 -8.664168500E-003 7.634703606E-003 5.176195250E-003 1.328265602E+001 -4.014662875E-003 7.090253278E-003 5.334923344E-003 1.337700371E+001 -5.563123725E-003 9.813827901E-003 7.842787275E-003 1.347135576E+001 -4.008706500E-003 9.679467491E-003 6.843190000E-003 1.356571216E+001 -5.680748125E-003 1.969109844E-003 1.548531125E-003 1.366007292E+001 -4.703063612E-003 3.798930391E-003 3.213598234E-003 1.375443804E+001 -2.227786625E-003 4.907187651E-003 4.217155531E-003 1.384880751E+001 -2.059825688E-003 7.038569806E-003 5.180280984E-003 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