# TOP STRAND, S1 # Oligo ID: R1447 # Sequence (5'to 3'): TTGTAGTCAT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R1448 # Sequence (5'to 3'): ATGACTACAA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: X9 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.0005 # [KCl] = 0.0 # [Tris] = 0.002 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 03-16-2004 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- 1.158514251E+001 8.181419125E-003 3.557691088E-003 2.678576875E-003 1.167941178E+001 6.382268875E-003 4.361276876E-003 3.391078125E-003 1.177368542E+001 4.337092010E-003 7.747775800E-003 4.793965492E-003 1.186796341E+001 1.807296775E-003 4.258009340E-003 3.635907975E-003 1.196224575E+001 3.450668000E-004 6.120446856E-003 4.493446550E-003 1.205653246E+001 1.785185924E-003 4.795625253E-003 3.425678307E-003 1.215082352E+001 2.732760188E-003 5.439029715E-003 3.203549953E-003 1.224511893E+001 8.415040000E-004 6.811955680E-003 5.451314000E-003 1.233941870E+001 -3.381294250E-003 5.187307170E-003 3.848991875E-003 1.243372283E+001 -6.383930000E-004 3.564538237E-003 2.671156000E-003 1.252803131E+001 4.523293987E-003 7.959316296E-003 6.444831509E-003 1.262234416E+001 1.062750314E-003 8.525955670E-003 6.320701936E-003 1.271666135E+001 -3.433860475E-003 5.500699471E-003 4.322568406E-003 1.281098291E+001 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8.702549750E-003 1.422632897E+001 -4.119261575E-003 5.995403521E-003 5.057819425E-003 1.432072023E+001 -3.419262250E-003 7.810508944E-003 5.221521500E-003 1.441511584E+001 -7.427553750E-005 3.575767245E-003 2.760057269E-003 1.450951581E+001 -1.894118375E-003 5.134347832E-003 4.088040375E-003 1.460392013E+001 -5.808354000E-004 9.106879712E-003 6.218985850E-003 1.469832881E+001 -3.574233000E-003 5.512347537E-003 4.237112250E-003 1.479274185E+001 -2.058255600E-003 7.010964907E-003 5.481619900E-003 1.488715924E+001 -4.614383250E-004 2.926186620E-003 2.239424756E-003 1.498158099E+001 -2.850760700E-003 5.897620810E-003 4.372292150E-003 1.507600710E+001 -2.111150500E-003 8.043689201E-003 5.790054625E-003 1.517043756E+001 6.215618750E-004 8.472047567E-003 6.846743906E-003 1.526487238E+001 4.023448347E-003 6.469991412E-003 5.307193153E-003 1.535931156E+001 1.972244950E-003 6.628147532E-003 5.164010800E-003 1.545375509E+001 -1.677840200E-003 4.935855311E-003 3.966146800E-003 1.554820298E+001 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