# TOP STRAND, S1 # Oligo ID: R1447 # Sequence (5'to 3'): TTGTAGTCAT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R1448 # Sequence (5'to 3'): ATGACTACAA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: Y9 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.003 # [KCl] = 0.0 # [Tris] = 0.002 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 04-11-2004 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- 1.413194207E+001 2.471678871E-002 3.269142382E-002 2.545936931E-002 1.422632897E+001 1.732797839E-002 2.771668330E-002 2.159886378E-002 1.432072023E+001 1.475008486E-002 2.777345642E-002 2.142028727E-002 1.441511584E+001 1.288771086E-002 3.377855013E-002 2.864552498E-002 1.450951581E+001 1.225894600E-002 2.398518420E-002 1.949281943E-002 1.460392013E+001 1.085700586E-002 2.075723988E-002 1.466858069E-002 1.469832881E+001 1.861492829E-002 1.349002642E-002 1.099819004E-002 1.479274185E+001 1.395127829E-002 2.708682898E-002 2.144821527E-002 1.488715924E+001 8.436168586E-003 1.052906936E-002 7.214310927E-003 1.498158099E+001 9.826520857E-003 1.551713509E-002 1.254697665E-002 1.507600710E+001 1.550273071E-002 2.305641676E-002 1.895603102E-002 1.517043756E+001 9.453750657E-003 2.154386859E-002 1.629811391E-002 1.526487238E+001 3.955111143E-003 1.827193674E-002 1.573382955E-002 1.535931156E+001 3.047308857E-003 1.820635062E-002 1.304706959E-002 1.545375509E+001 1.934370429E-003 2.113841999E-002 1.667261249E-002 1.554820298E+001 4.683122657E-003 2.602045480E-002 2.035734420E-002 1.564265522E+001 7.515262757E-003 1.018303059E-002 8.662137637E-003 1.573711182E+001 5.666865714E-003 8.381020527E-003 6.924775102E-003 1.583157278E+001 3.996941571E-003 1.071437573E-002 7.616866939E-003 1.592603809E+001 1.198823871E-002 1.840781189E-002 1.461945216E-002 1.602050776E+001 -5.164492286E-003 1.243472898E-002 9.205295184E-003 1.611498179E+001 2.741404000E-003 1.864015093E-002 1.253103371E-002 1.620946017E+001 9.165142143E-003 1.492394557E-002 1.223746102E-002 1.630394291E+001 6.024221714E-003 9.281901877E-003 7.475078898E-003 1.639843000E+001 4.855758714E-003 1.149238364E-002 9.810255388E-003 1.649292146E+001 -1.534625000E-003 1.324737094E-002 1.125812714E-002 1.658741726E+001 -1.463455143E-003 1.136143800E-002 8.787713265E-003 1.668191743E+001 -1.220067014E-002 1.216041670E-002 1.047967702E-002 1.677642195E+001 -5.519107286E-003 1.066255595E-002 7.750659388E-003 1.687093083E+001 -6.910515514E-003 1.295862516E-002 1.063339242E-002 1.696544406E+001 5.343237571E-003 4.844838514E-003 3.313387633E-003 1.705996165E+001 8.984908143E-003 3.709719363E-003 3.067473020E-003 1.715448360E+001 1.888129687E-003 7.682200472E-003 5.241308929E-003 1.724900990E+001 9.744173629E-003 1.310767648E-002 9.263036890E-003 1.734354056E+001 -3.137763771E-003 1.177673531E-002 9.039527118E-003 1.743807557E+001 2.713665429E-003 8.722814400E-003 7.895156367E-003 1.753261494E+001 -1.366009843E-003 1.318454701E-002 1.002490442E-002 1.762715867E+001 6.460338971E-003 1.427795887E-002 9.048448310E-003 1.772170676E+001 -6.841998429E-004 9.908639762E-003 7.566360751E-003 1.781625920E+001 -1.593155286E-003 9.096294065E-003 7.629616816E-003 1.791081599E+001 -4.646130000E-004 1.607172585E-002 1.156036571E-002 1.800537715E+001 -2.656972000E-003 1.546800054E-002 1.027436829E-002 1.809994266E+001 4.179341914E-003 2.021719609E-002 1.606199324E-002 1.819451252E+001 1.207592229E-003 1.774461809E-002 1.382252780E-002 1.828908675E+001 6.122882143E-003 1.610104979E-002 1.032174649E-002 1.838366533E+001 -1.307983571E-003 1.413174959E-002 9.081474939E-003 1.847824826E+001 2.389396343E-003 1.475882055E-002 1.079704904E-002 1.857283555E+001 6.260345714E-004 2.136526553E-002 1.578856763E-002 1.866742720E+001 -1.731459000E-004 1.938653080E-002 1.264596794E-002 1.876202321E+001 6.759568071E-003 2.326731302E-002 1.772516110E-002 1.885662357E+001 4.112990143E-003 2.551183574E-002 2.144057131E-002 1.895122828E+001 4.229644671E-003 1.917151978E-002 1.475869495E-002 1.904583736E+001 1.032572829E-002 2.632549818E-002 2.110563576E-002 1.914045079E+001 1.226041229E-002 1.454047659E-002 9.679623755E-003 1.923506857E+001 9.674982143E-003 1.638851920E-002 1.211483245E-002 1.932969072E+001 9.375815743E-003 1.602408626E-002 1.148556801E-002 1.942431721E+001 6.851352929E-003 2.657470629E-002 1.876840606E-002 1.951894807E+001 5.378896714E-003 2.666946166E-002 2.066518347E-002 1.961358328E+001 2.414722571E-003 2.877205047E-002 2.254109494E-002 1.970822285E+001 5.082658986E-003 2.102779616E-002 1.420928915E-002 1.980286677E+001 2.591453286E-003 2.618837898E-002 2.146327996E-002 1.989751505E+001 1.633543893E-002 1.985193297E-002 1.728569235E-002 1.999216769E+001 7.106613571E-003 2.372505766E-002 1.893668939E-002 2.008682469E+001 1.020953071E-002 2.635375118E-002 1.981476510E-002 2.018148604E+001 3.393886371E-002 2.373794210E-002 1.635402433E-002 2.027615174E+001 2.096383574E-002 2.320637021E-002 1.698408201E-002 2.037082180E+001 1.211413629E-002 2.661023105E-002 2.134243747E-002 2.046549622E+001 2.411770731E-002 1.923128559E-002 1.388252593E-002 2.056017500E+001 2.348602714E-002 1.338680778E-002 1.097902531E-002 2.065485813E+001 1.783711343E-002 1.980652967E-002 1.662109992E-002 2.074954562E+001 2.134555831E-002 1.931195835E-002 1.618659336E-002 2.084423746E+001 2.278534014E-002 2.629871057E-002 1.885849702E-002 2.093893366E+001 2.161756929E-002 2.915844526E-002 2.097775796E-002 2.103363422E+001 2.316573914E-002 3.102216535E-002 2.044340049E-002 2.112833913E+001 2.289570394E-002 1.998297556E-002 1.469218918E-002 2.122304840E+001 2.103492157E-002 2.377006492E-002 1.962384437E-002 2.131776203E+001 3.193349714E-002 1.992393564E-002 1.612239102E-002 2.141248001E+001 2.843740900E-002 2.531433342E-002 1.916382914E-002 2.150720235E+001 2.477453917E-002 2.300680925E-002 1.849685785E-002 2.160192905E+001 3.176260000E-002 2.267870002E-002 1.695735714E-002 2.169666010E+001 3.203495900E-002 2.313298069E-002 1.588817771E-002 2.179139551E+001 3.144112500E-002 2.589026934E-002 1.795538000E-002 2.188613527E+001 3.402676343E-002 2.481735543E-002 1.738108563E-002 2.198087939E+001 3.593850857E-002 1.648824048E-002 1.324857878E-002 2.207562787E+001 4.560147286E-002 2.380721613E-002 1.603014408E-002 2.217038070E+001 4.071086671E-002 3.146222917E-002 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6.056159286E-002 3.002034684E-002 2.076114898E-002 2.368701850E+001 7.343011714E-002 2.675362284E-002 2.008058245E-002 2.378184540E+001 7.259487143E-002 3.119843579E-002 2.417267347E-002 2.387667664E+001 7.130067714E-002 3.503066767E-002 2.580847755E-002 2.397151225E+001 7.349491714E-002 3.213734427E-002 2.099380816E-002 2.406635221E+001 7.796917143E-002 3.973834011E-002 2.555096449E-002 2.416119653E+001 7.410886857E-002 3.778343958E-002 2.432561020E-002 2.425604520E+001 8.658627143E-002 2.915181162E-002 2.217031306E-002 2.435089823E+001 7.974005857E-002 4.033255982E-002 2.605178653E-002 2.444575562E+001 9.658006286E-002 3.801082767E-002 2.970907837E-002 2.454061736E+001 8.728224429E-002 3.322585351E-002 2.426837918E-002 2.463548346E+001 8.727266571E-002 4.184200348E-002 3.293908653E-002 2.473035391E+001 8.249987429E-002 4.451626300E-002 3.408410367E-002 2.482522873E+001 9.211193000E-002 4.237816307E-002 3.054660000E-002 2.492010789E+001 9.758643429E-002 3.928792956E-002 2.756899918E-002 2.501499142E+001 1.030951029E-001 3.532438279E-002 2.596382694E-002 2.510987930E+001 1.016865429E-001 4.277730711E-002 2.689557551E-002 2.520477154E+001 1.017020800E-001 4.451063294E-002 3.523141714E-002 2.529966813E+001 1.061807629E-001 4.260314491E-002 3.510600327E-002 2.539456908E+001 1.065217100E-001 3.510867650E-002 2.425139429E-002 2.548947439E+001 1.106885614E-001 4.348972861E-002 2.781586163E-002 2.558438405E+001 1.158907814E-001 4.663113135E-002 3.181422122E-002 2.567929807E+001 1.091368814E-001 4.370464262E-002 2.852039265E-002 2.577421644E+001 1.259377571E-001 4.191167327E-002 2.759817959E-002 2.586913917E+001 1.209047586E-001 3.742804975E-002 2.503059020E-002 2.596406626E+001 1.240646986E-001 3.919042157E-002 2.976140163E-002 2.605899771E+001 1.431000971E-001 4.630563650E-002 3.383874612E-002 2.615393351E+001 1.235433214E-001 4.626685666E-002 3.850329592E-002 2.624887366E+001 1.425110743E-001 4.972420058E-002 3.533636490E-002 2.634381818E+001 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