# TOP STRAND, S1 # Oligo ID: R1447 # Sequence (5'to 3'): TTGTAGTCAT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R1448 # Sequence (5'to 3'): ATGACTACAA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: Z9 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.020 # [KCl] = 0.0 # [Tris] = 0.002 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 05-18-2004 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- 1.517043756E+001 4.785112000E-003 4.476830488E-003 3.234407500E-003 1.526487238E+001 3.907195750E-003 4.812758745E-003 3.145223375E-003 1.535931156E+001 2.487724125E-003 4.466245926E-003 3.296587375E-003 1.545375509E+001 3.404913500E-003 3.937950463E-003 3.306358875E-003 1.554820298E+001 1.502270888E-003 5.284304254E-003 3.812436613E-003 1.564265522E+001 2.249667450E-003 4.850953499E-003 4.003026913E-003 1.573711182E+001 -1.467049638E-003 1.414890790E-003 1.224743613E-003 1.583157278E+001 -5.094097250E-004 4.385642544E-003 3.078704275E-003 1.592603809E+001 3.190573625E-004 3.846672675E-003 2.518827247E-003 1.602050776E+001 5.328765250E-004 3.287668057E-003 2.330758350E-003 1.611498179E+001 2.872753375E-003 3.948332279E-003 2.743662875E-003 1.620946017E+001 -2.125008900E-003 3.181038994E-003 2.599380825E-003 1.630394291E+001 -2.789790250E-004 3.157048587E-003 2.502208019E-003 1.639843000E+001 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2.295959050E-003 1.781625920E+001 1.045498185E-003 3.939137487E-003 3.282535111E-003 1.791081599E+001 -1.140909147E-003 5.226915247E-003 3.990571140E-003 1.800537715E+001 -3.855300137E-003 5.017334277E-003 4.051537362E-003 1.809994266E+001 -1.881563262E-003 2.621505581E-003 2.036644237E-003 1.819451252E+001 5.271065587E-004 4.049360105E-003 2.976099666E-003 1.828908675E+001 -2.939650000E-005 5.980466114E-003 4.451744125E-003 1.838366533E+001 -1.939438750E-004 4.751702282E-003 4.102951875E-003 1.847824826E+001 -3.679660025E-003 3.834044807E-003 3.154285975E-003 1.857283555E+001 -7.949061750E-004 1.899523130E-003 1.468893369E-003 1.866742720E+001 5.644132750E-004 2.483745253E-003 2.144316225E-003 1.876202321E+001 -2.576098400E-003 4.061119043E-003 3.349444850E-003 1.885662357E+001 6.026115375E-004 3.274419172E-003 2.465654903E-003 1.895122828E+001 -4.307131250E-004 3.902738013E-003 2.968901975E-003 1.904583736E+001 -7.241609250E-004 3.160425128E-003 2.554119075E-003 1.914045079E+001 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