# TOP STRAND, S1 # Oligo ID: R1453 # Sequence (5'to 3'): GATGCGCTCG # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R1454 # Sequence (5'to 3'): CGAGCGCATC # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: D1 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.125 # [KCl] = 0.0 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 02-20-2004 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- 1.602050776E+001 -1.493152200E-002 2.406119096E-002 2.064403800E-002 1.611498179E+001 -1.916630283E-002 1.655986337E-002 1.369778218E-002 1.620946017E+001 -1.291411500E-002 2.683858524E-002 2.322554000E-002 1.630394291E+001 -1.588192825E-002 2.551584275E-002 2.158151175E-002 1.639843000E+001 -1.604650058E-002 1.880516751E-002 1.603361942E-002 1.649292146E+001 -1.684948250E-002 1.720726844E-002 1.440753750E-002 1.658741726E+001 -1.779318750E-002 1.521679815E-002 1.296739750E-002 1.668191743E+001 -1.391433465E-002 1.669365266E-002 1.441305535E-002 1.677642195E+001 -1.240653898E-002 1.249393580E-002 1.068639603E-002 1.687093083E+001 -1.420499875E-002 1.540835905E-002 1.274934625E-002 1.696544406E+001 -1.324589225E-002 1.325325844E-002 1.111112275E-002 1.705996165E+001 -1.368051325E-002 1.220689996E-002 9.794806750E-003 1.715448360E+001 -1.562190425E-002 1.266009823E-002 1.021496075E-002 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