# TOP STRAND, S1 # Oligo ID: R1453 # Sequence (5'to 3'): GATGCGCTCG # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R1454 # Sequence (5'to 3'): CGAGCGCATC # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: Q9 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.0015 # [KCl] = 0.0 # [Tris] = 0.002 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 03-30-2004 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- 1.592603809E+001 -6.350457250E-003 4.731696127E-003 3.918031750E-003 1.602050776E+001 -8.373502250E-003 1.093914442E-002 9.147437750E-003 1.611498179E+001 5.634110250E-003 9.932135217E-003 8.209484750E-003 1.620946017E+001 -4.454641750E-003 1.615775980E-002 1.167739088E-002 1.630394291E+001 -5.272876500E-003 7.223976004E-003 5.119529750E-003 1.639843000E+001 -7.095820250E-003 1.557935387E-002 1.207602975E-002 1.649292146E+001 -1.314021150E-002 1.240536566E-002 9.992263500E-003 1.658741726E+001 -9.421636000E-003 8.414144145E-003 6.183332000E-003 1.668191743E+001 -4.424472750E-003 1.269482415E-002 8.593569750E-003 1.677642195E+001 -5.808294000E-003 1.287616193E-002 9.358237000E-003 1.687093083E+001 -8.685333000E-003 5.776768137E-003 4.296637000E-003 1.696544406E+001 -4.630283250E-003 2.066294042E-003 1.547026750E-003 1.705996165E+001 -6.155173500E-003 3.867167141E-003 2.782778250E-003 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