# TOP STRAND, S1 # Oligo ID: R1453 # Sequence (5'to 3'): GATGCGCTCG # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R1454 # Sequence (5'to 3'): CGAGCGCATC # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: R9 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.010 # [KCl] = 0.0 # [Tris] = 0.002 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 04-27-2004 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- 1.045425098E+001 1.972832050E-003 7.349589148E-003 5.219215700E-003 1.054846798E+001 1.528212000E-003 7.144881572E-003 5.193869250E-003 1.064268934E+001 2.110430625E-003 6.957633328E-003 5.529440719E-003 1.073691505E+001 9.345806250E-004 5.164476233E-003 4.346910469E-003 1.083114512E+001 4.346407375E-003 7.135874816E-003 5.290307625E-003 1.092537955E+001 1.174176625E-003 6.653015530E-003 4.946896469E-003 1.101961833E+001 4.022412750E-003 8.124157906E-003 6.869170750E-003 1.111386147E+001 4.227878487E-003 6.469860645E-003 4.809258384E-003 1.120810896E+001 3.570468250E-003 6.964315388E-003 5.006398375E-003 1.130236081E+001 2.548873775E-003 5.372820423E-003 3.836672975E-003 1.139661702E+001 2.540244546E-003 5.514521101E-003 4.069015954E-003 1.149087759E+001 3.672735350E-003 6.058066052E-003 4.505337325E-003 1.158514251E+001 3.008976125E-003 7.390146981E-003 6.031515594E-003 1.167941178E+001 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