# TOP STRAND, S1 # Oligo ID: R1453 # Sequence (5'to 3'): GATGCGCTCG # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R1454 # Sequence (5'to 3'): CGAGCGCATC # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: V1 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.050 # [KCl] = 0.0 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 02-21-2004 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- 1.649292146E+001 -8.690961500E-003 1.413402660E-002 1.029514387E-002 1.658741726E+001 -5.480465500E-003 9.979025199E-003 7.599227125E-003 1.668191743E+001 -9.106593375E-003 1.248293469E-002 1.103033412E-002 1.677642195E+001 -6.376540125E-003 1.314591467E-002 1.086531238E-002 1.687093083E+001 -3.082107250E-003 1.618298596E-002 1.427910275E-002 1.696544406E+001 -1.017383850E-002 8.328489822E-003 6.845141500E-003 1.705996165E+001 -8.857376750E-003 1.303875341E-002 9.760167813E-003 1.715448360E+001 -3.052601875E-003 1.391055998E-002 1.063360337E-002 1.724900990E+001 -1.112742785E-002 8.386348623E-003 7.052349650E-003 1.734354056E+001 -8.143231375E-003 7.392440289E-003 6.414788281E-003 1.743807557E+001 -7.712747450E-003 9.497899591E-003 8.248152550E-003 1.753261494E+001 -9.791866500E-003 1.110168589E-002 8.696693500E-003 1.762715867E+001 -1.060531625E-002 1.293918325E-002 8.771709688E-003 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