# TOP STRAND, S1 # Oligo ID: R1453 # Sequence (5'to 3'): GATGCGCTCG # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R1454 # Sequence (5'to 3'): CGAGCGCATC # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: X9 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.0005 # [KCl] = 0.0 # [Tris] = 0.002 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 03-16-2004 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- 1.158514251E+001 1.053853900E-002 9.152677994E-003 6.043646500E-003 1.167941178E+001 1.787380725E-002 1.429531492E-002 1.015831775E-002 1.177368542E+001 1.399174440E-002 1.135841253E-002 7.932365600E-003 1.186796341E+001 1.083970157E-002 8.480206629E-003 5.220449212E-003 1.196224575E+001 4.411958750E-003 9.370519954E-003 7.988436250E-003 1.205653246E+001 1.439557625E-002 1.442102230E-002 1.034076875E-002 1.215082352E+001 1.020772425E-002 8.385089707E-003 6.016323625E-003 1.224511893E+001 1.111392925E-002 8.721273578E-003 6.188805750E-003 1.233941870E+001 8.003100500E-003 9.542056412E-003 7.452284500E-003 1.243372283E+001 1.470903275E-002 1.411631317E-002 9.526190875E-003 1.252803131E+001 1.159742560E-002 9.758988194E-003 6.837359400E-003 1.262234416E+001 1.041353875E-002 6.091816430E-003 4.352035625E-003 1.271666135E+001 4.844561950E-003 9.752920213E-003 7.750473550E-003 1.281098291E+001 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