# TOP STRAND, S1 # Oligo ID: R1453 # Sequence (5'to 3'): GATGCGCTCG # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R1454 # Sequence (5'to 3'): CGAGCGCATC # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: Y9 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.003 # [KCl] = 0.0 # [Tris] = 0.002 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 04-10-2004 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- 1.073691505E+001 -5.589653750E-003 5.149732969E-002 3.760715031E-002 1.083114512E+001 6.984486875E-003 5.097402524E-002 3.585574488E-002 1.092537955E+001 7.878200125E-003 4.615761995E-002 3.123425091E-002 1.101961833E+001 3.713046137E-003 4.675472697E-002 3.222523190E-002 1.111386147E+001 -3.336222013E-003 3.908955191E-002 2.784243399E-002 1.120810896E+001 3.078021112E-003 4.779831528E-002 3.465778917E-002 1.130236081E+001 2.778608250E-003 4.049099274E-002 2.951951875E-002 1.139661702E+001 1.152384750E-002 3.606619003E-002 2.876939937E-002 1.149087759E+001 1.023012838E-002 3.579870961E-002 2.708051163E-002 1.158514251E+001 8.559535000E-003 2.620690195E-002 2.055022500E-002 1.167941178E+001 5.964726750E-003 3.112284593E-002 2.460357244E-002 1.177368542E+001 5.648705000E-003 3.900112488E-002 2.937606000E-002 1.186796341E+001 -4.443562500E-004 2.883042926E-002 2.425322875E-002 1.196224575E+001 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