# TOP STRAND, S1 # Oligo ID: R1453 # Sequence (5'to 3'): GATGCGCTCG # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R1454 # Sequence (5'to 3'): CGAGCGCATC # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: Z9 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.020 # [KCl] = 0.0 # [Tris] = 0.002 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 05-18-2004 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- 1.583157278E+001 -2.099857200E-004 5.232446886E-003 3.687284780E-003 1.592603809E+001 -4.324367500E-003 5.011420661E-003 3.875576500E-003 1.602050776E+001 -3.065980990E-003 8.609610153E-003 6.023230010E-003 1.611498179E+001 -3.344701500E-003 5.728861464E-003 3.995277250E-003 1.620946017E+001 -3.520448750E-003 6.662699696E-003 5.182150250E-003 1.630394291E+001 -1.087448825E-003 5.433240416E-003 4.296487175E-003 1.639843000E+001 -1.367951025E-003 5.549396660E-003 3.774400475E-003 1.649292146E+001 1.717680875E-003 6.582262501E-003 4.859254125E-003 1.658741726E+001 1.796749500E-003 6.634563327E-003 5.234759000E-003 1.668191743E+001 -8.918321500E-004 2.365722191E-003 1.711302925E-003 1.677642195E+001 -2.015710000E-003 5.874718008E-003 4.360546000E-003 1.687093083E+001 -1.613186375E-003 2.530213722E-003 2.158547625E-003 1.696544406E+001 -2.047291750E-003 1.001502994E-002 8.042559250E-003 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