# TOP STRAND, S1 # Oligo ID: R727 # Sequence (5'to 3'): TGATTCTACCTATGTGATTT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R730 # Sequence (5'to 3'): AAATCACATAGGTAGAATCA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: D1 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.125 # [KCl] = 0.0 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 11-05-2003 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1590000000E+02 .1138717877E-01 .1063464745E-01 .9155041225E-02 .1600000000E+02 .9439404300E-02 .1199559528E-01 .1031456070E-01 .1610000000E+02 .1065462325E-01 .1197007464E-01 .1002663675E-01 .1620000000E+02 .1058113458E-01 .1284141236E-01 .1107154043E-01 .1630000000E+02 .1091212825E-01 .1298459196E-01 .1098636675E-01 .1640000000E+02 .1043335100E-01 .1116493132E-01 .8951384000E-02 .1650000000E+02 .9389510500E-02 .9732157490E-02 .8103239500E-02 .1660000000E+02 .9889776100E-02 .1127477941E-01 .9514928900E-02 .1670000000E+02 .7457629750E-02 .1125592633E-01 .9322180250E-02 .1680000000E+02 .1290332188E-01 .1260397575E-01 .1072935313E-01 .1690000000E+02 .8475670850E-02 .9867306063E-02 .8321374150E-02 .1700000000E+02 .1016814125E-01 .8524498862E-02 .5721913750E-02 .1710000000E+02 .1180390725E-01 .1092802771E-01 .9149677750E-02 .1720000000E+02 .1197777007E-01 .9362702366E-02 .6338259888E-02 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