# TOP STRAND, S1 # Oligo ID: R727 # Sequence (5'to 3'): TGATTCTACCTATGTGATTT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R730 # Sequence (5'to 3'): AAATCACATAGGTAGAATCA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: H1 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.300 # [KCl] = 0.0 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 09-20-2004 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1700000000E+02 .8327309000E-02 .5612411519E-02 .4003663000E-02 .1710000000E+02 .8409358962E-02 .6344154084E-02 .4754519787E-02 .1720000000E+02 .7831217875E-02 .6218353512E-02 .4860826625E-02 .1730000000E+02 .7700394037E-02 .6075840983E-02 .5223646972E-02 .1740000000E+02 .7706666800E-02 .5811975875E-02 .5009454900E-02 .1750000000E+02 .8355118625E-02 .6195948658E-02 .4905308531E-02 .1760000000E+02 .6827805125E-02 .5268974726E-02 .4531758656E-02 .1770000000E+02 .7534314625E-02 .5967836130E-02 .5044141531E-02 .1780000000E+02 .7277610615E-02 .5828897511E-02 .4932072885E-02 .1790000000E+02 .7826699625E-02 .4534254745E-02 .3941071125E-02 .1800000000E+02 .7449780388E-02 .4103834847E-02 .3084109709E-02 .1810000000E+02 .5993142275E-02 .5794063694E-02 .4576619406E-02 .1820000000E+02 .7464099750E-02 .5632965787E-02 .4399441688E-02 .1830000000E+02 .6749066363E-02 .6419278771E-02 .5114587728E-02 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