# TOP STRAND, S1 # Oligo ID: R727 # Sequence (5'to 3'): TGATTCTACCTATGTGATTT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R730 # Sequence (5'to 3'): AAATCACATAGGTAGAATCA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: N1 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.600 # [KCl] = 0.0 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 09-23-2004 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1610000000E+02 .8655980000E-03 .4949929061E-02 .3440753500E-02 .1620000000E+02 .5467432500E-03 .5998283755E-02 .4872680250E-02 .1630000000E+02 .2562453250E-02 .6687355896E-02 .4685548750E-02 .1640000000E+02 .2214611500E-02 .7005219082E-02 .4856590750E-02 .1650000000E+02 .2040480500E-02 .6922571374E-02 .5040803250E-02 .1660000000E+02 .8264920000E-03 .5468801090E-02 .3975199500E-02 .1670000000E+02 .1276742175E-02 .4597018736E-02 .3364506413E-02 .1680000000E+02 .6977053500E-03 .4275156543E-02 .3106868650E-02 .1690000000E+02 -.1056195100E-02 .6946826421E-02 .4911586400E-02 .1700000000E+02 -.2385495750E-02 .6591234602E-02 .4875098250E-02 .1710000000E+02 -.1967714250E-02 .6278529277E-02 .4089018250E-02 .1720000000E+02 -.4134325000E-03 .6514304918E-02 .5024588500E-02 .1730000000E+02 -.3947619000E-03 .3009628675E-02 .2097674050E-02 .1740000000E+02 .1199770000E-03 .7166127084E-02 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