# TOP STRAND, S1 # Oligo ID: R727 # Sequence (5'to 3'): TGATTCTACCTATGTGATTT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R730 # Sequence (5'to 3'): AAATCACATAGGTAGAATCA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: V1 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.050 # [KCl] = 0.0 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 09-25-2003 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1710000000E+02 .7859021750E-02 .1031215622E-01 .8724083250E-02 .1720000000E+02 .7070624750E-02 .1129086899E-01 .9598435250E-02 .1730000000E+02 .6215764250E-02 .1192537224E-01 .1007438075E-01 .1740000000E+02 .6139730250E-02 .1000393316E-01 .8472484750E-02 .1750000000E+02 .7177299725E-02 .9468049924E-02 .7865810275E-02 .1760000000E+02 .5753609500E-02 .1177886036E-01 .1002278550E-01 .1770000000E+02 .8395562097E-02 .1075099072E-01 .9243537902E-02 .1780000000E+02 .4566837750E-02 .8533343975E-02 .7355237250E-02 .1790000000E+02 .6755169000E-02 .9677167534E-02 .8250216000E-02 .1800000000E+02 .6235585275E-02 .9499726451E-02 .7675264725E-02 .1810000000E+02 .2889274000E-02 .8995002638E-02 .7679878000E-02 .1820000000E+02 .5084942125E-02 .9252374926E-02 .7551229375E-02 .1830000000E+02 .4098064250E-02 .9901385238E-02 .8004279750E-02 .1840000000E+02 .4981115250E-02 .1073482473E-01 .8963174750E-02 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