# TOP STRAND, S1 # Oligo ID: R727 # Sequence (5'to 3'): TGATTCTACCTATGTGATTT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R730 # Sequence (5'to 3'): AAATCACATAGGTAGAATCA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: Y9 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.003 # [KCl] = 0.0 # [Tris] = 0.002 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 04-01-2004 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1530000000E+02 .3804340038E-02 .6820738191E-02 .5891746712E-02 .1540000000E+02 .3308359537E-02 .6232954628E-02 .4928015213E-02 .1550000000E+02 .2991180125E-02 .6569862821E-02 .5320147875E-02 .1560000000E+02 .3280057938E-02 .5636251137E-02 .4699743063E-02 .1570000000E+02 .3784294250E-02 .5423272245E-02 .3957767750E-02 .1580000000E+02 .4019581238E-02 .6346567661E-02 .5436759013E-02 .1590000000E+02 .2695048638E-02 .7527278978E-02 .5973567613E-02 .1600000000E+02 .2800772750E-02 .6680768906E-02 .5423068000E-02 .1610000000E+02 .3258596875E-02 .6094666465E-02 .4697814406E-02 .1620000000E+02 .1980033750E-02 .6942667008E-02 .5720279500E-02 .1630000000E+02 .2019206250E-02 .7419890729E-02 .5574674812E-02 .1640000000E+02 .1341815875E-02 .7825774939E-02 .6254880375E-02 .1650000000E+02 .3452680400E-02 .6644588000E-02 .5210962750E-02 .1660000000E+02 .3681056375E-02 .6545785390E-02 .5195480281E-02 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