# TOP STRAND, S1 # Oligo ID: R728 # Sequence (5'to 3'): AGCTGCAGTGGATGTGAGAA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R731 # Sequence (5'to 3'): TTCTCACATCCACTGCAGCT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: Q9 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.0015 # [KCl] = 0.0 # [Tris] = 0.002 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 03-19-2004 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1180000000E+02 -.1126332750E-01 .2626661397E-01 .2065194750E-01 .1190000000E+02 -.1104277363E-01 .2735668036E-01 .2127215387E-01 .1200000000E+02 -.1142943100E-01 .2601608412E-01 .2065252650E-01 .1210000000E+02 -.1162662075E-01 .2668546113E-01 .2058468944E-01 .1220000000E+02 -.1250526488E-01 .2668042965E-01 .2159354762E-01 .1230000000E+02 -.1172173037E-01 .2727561256E-01 .2125804212E-01 .1240000000E+02 -.1050602012E-01 .2670171482E-01 .2086594737E-01 .1250000000E+02 -.1154456237E-01 .2745109919E-01 .2143882512E-01 .1260000000E+02 -.1082723125E-01 .2610455946E-01 .2017683375E-01 .1270000000E+02 -.9634729000E-02 .2782692044E-01 .2195425850E-01 .1280000000E+02 -.1058817787E-01 .2782327471E-01 .2150386712E-01 .1290000000E+02 -.1098796012E-01 .2714277904E-01 .2098480737E-01 .1300000000E+02 -.1023586050E-01 .2649908060E-01 .2089800712E-01 .1310000000E+02 -.1062652650E-01 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