# TOP STRAND, S1 # Oligo ID: R728 # Sequence (5'to 3'): AGCTGCAGTGGATGTGAGAA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R731 # Sequence (5'to 3'): TTCTCACATCCACTGCAGCT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: Y9 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.003 # [KCl] = 0.0 # [Tris] = 0.002 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 04-05-2004 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1530000000E+02 -.1242052557E-01 .1484267482E-01 .1265977442E-01 .1540000000E+02 -.1202122180E-01 .1566753116E-01 .1323562820E-01 .1550000000E+02 -.1198571525E-01 .1469459788E-01 .1235240475E-01 .1560000000E+02 -.1123859900E-01 .1481798586E-01 .1238390600E-01 .1570000000E+02 -.1028279250E-01 .1419291344E-01 .1152404750E-01 .1580000000E+02 -.1153984038E-01 .1402127398E-01 .1202894462E-01 .1590000000E+02 -.9608075750E-02 .1138737713E-01 .9398379250E-02 .1600000000E+02 -.1121815250E-01 .1390829455E-01 .1141493250E-01 .1610000000E+02 -.1120930368E-01 .1547028022E-01 .1228925633E-01 .1620000000E+02 -.9770333725E-02 .1457702264E-01 .1116042128E-01 .1630000000E+02 -.9650788150E-02 .1444398459E-01 .1172691685E-01 .1640000000E+02 -.1002890325E-01 .1470100887E-01 .1221252675E-01 .1650000000E+02 -.8801277500E-02 .1433879823E-01 .1103376250E-01 .1660000000E+02 -.8539305050E-02 .1367731221E-01 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