# TOP STRAND, S1 # Oligo ID: R779 # Sequence (5'to 3'): CAGCCTCGTCGCAGC # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R782 # Sequence (5'to 3'): GCTGCGACGAGGCTG # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: H1 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.300 # [KCl] = 0.0 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 09-20-2004 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted 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