# TOP STRAND, S1 # Oligo ID: R779 # Sequence (5'to 3'): CAGCCTCGTCGCAGC # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R782 # Sequence (5'to 3'): GCTGCGACGAGGCTG # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: Z9 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.020 # [KCl] = 0.0 # [Tris] = 0.002 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 04-30-2004 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1940000000E+02 .1364303500E-01 .2784876514E-01 .1849347750E-01 .1950000000E+02 .1292537700E-01 .2609797060E-01 .1805905300E-01 .1960000000E+02 .1399956500E-01 .2732227613E-01 .1827795750E-01 .1970000000E+02 .1484678000E-01 .2195718102E-01 .1379726500E-01 .1980000000E+02 .1270697250E-01 .2748069668E-01 .1951809625E-01 .1990000000E+02 .1160947500E-01 .2689668672E-01 .1809946250E-01 .2000000000E+02 .8474127000E-02 .2914021459E-01 .1911095850E-01 .2010000000E+02 .1070330000E-01 .2360613190E-01 .1512129500E-01 .2020000000E+02 .1233237000E-01 .1875835535E-01 .1260428500E-01 .2030000000E+02 .1250037500E-01 .2333208149E-01 .1563787750E-01 .2040000000E+02 .1002217900E-01 .2805013982E-01 .1885895100E-01 .2050000000E+02 .1216726500E-01 .2477531457E-01 .1572835750E-01 .2060000000E+02 .1215597000E-01 .2189416014E-01 .1420265500E-01 .2070000000E+02 .1166338000E-01 .2478458158E-01 .1607437500E-01 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