# TOP STRAND, S1 # Oligo ID: R777 # Sequence (5'to 3'): TTCTACCTATGTGAT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R780 # Sequence (5'to 3'): ATCACATAGGTAGAA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: D5 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.125 # [KCl] = 0.200 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 11-22-2003 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1430000000E+02 .3444458500E-02 .5426579039E-02 .3913446375E-02 .1440000000E+02 .3472247564E-02 .5192981639E-02 .4566223936E-02 .1450000000E+02 .1370854663E-02 .5048127165E-02 .4157211003E-02 .1460000000E+02 .3425164912E-02 .2916125820E-02 .2229530588E-02 .1470000000E+02 .4318232500E-02 .6418485423E-02 .4970868875E-02 .1480000000E+02 .1421780250E-02 .4042537307E-02 .3428824687E-02 .1490000000E+02 .2772448305E-02 .3934767877E-02 .3539862195E-02 .1500000000E+02 .3957006275E-02 .3892221504E-02 .2863249794E-02 .1510000000E+02 .3415256962E-02 .4187357023E-02 .3331219028E-02 .1520000000E+02 .7660076250E-03 .3267694732E-02 .2816138969E-02 .1530000000E+02 .4583815613E-02 .4592454412E-02 .3926568791E-02 .1540000000E+02 .4526262625E-02 .5526202764E-02 .3819735937E-02 .1550000000E+02 .3524362735E-02 .5390785143E-02 .4339786515E-02 .1560000000E+02 .2572186098E-02 .4870509777E-02 .3615124653E-02 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