# TOP STRAND, S1 # Oligo ID: R777 # Sequence (5'to 3'): TTCTACCTATGTGAT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R780 # Sequence (5'to 3'): ATCACATAGGTAGAA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: D7 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.125 # [KCl] = 1.000 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 12-07-2003 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1420000000E+02 -.2573356125E-02 .3273108931E-02 .2604967094E-02 .1430000000E+02 -.1712624212E-02 .4962862379E-02 .3829514787E-02 .1440000000E+02 -.1188607500E-03 .5562025885E-02 .4346622063E-02 .1450000000E+02 -.1410024925E-02 .2814042558E-02 .1997557825E-02 .1460000000E+02 -.1174513100E-02 .5615888262E-02 .3644451875E-02 .1470000000E+02 -.2205490625E-02 .2922982738E-02 .2069545031E-02 .1480000000E+02 -.1047464025E-02 .3261566632E-02 .2354504225E-02 .1490000000E+02 .1467316650E-02 .4250970243E-02 .3462796850E-02 .1500000000E+02 -.3493591300E-03 .2583046277E-02 .1901347778E-02 .1510000000E+02 -.1353242475E-02 .4440317516E-02 .3714489394E-02 .1520000000E+02 .2872318625E-03 .4395752610E-02 .3340043703E-02 .1530000000E+02 -.1810596250E-03 .3827199917E-02 .2542096319E-02 .1540000000E+02 -.8131796250E-03 .3221668432E-02 .2583916719E-02 .1550000000E+02 -.3310618750E-03 .7349249393E-02 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