# TOP STRAND, S1 # Oligo ID: R777 # Sequence (5'to 3'): TTCTACCTATGTGAT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R780 # Sequence (5'to 3'): ATCACATAGGTAGAA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: P5 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.0015 # [KCl] = 0.050 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 08-28-2002 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- "BLANK file: p5avb02.DTA DATE: 08-27-2002 TIME: 09:39:07" 1.611498179E+001 -4.423109250E-003 6.478807546E-003 5.294264750E-003 1.620946017E+001 -4.591463563E-003 6.225498375E-003 5.256831578E-003 1.630394291E+001 -3.169594850E-003 5.421802034E-003 4.388509363E-003 1.639843000E+001 -2.144053875E-003 5.641242478E-003 4.690222594E-003 1.649292146E+001 -4.111041400E-003 5.282646228E-003 4.064112850E-003 1.658741726E+001 -2.345928246E-003 4.738870811E-003 3.800004565E-003 1.668191743E+001 -2.065570125E-003 4.282567083E-003 3.698697156E-003 1.677642195E+001 -1.931545275E-003 4.666375780E-003 4.142732294E-003 1.687093083E+001 -1.829739627E-003 4.887630408E-003 3.886117779E-003 1.696544406E+001 -3.093442554E-003 4.077656687E-003 3.446084585E-003 1.705996165E+001 -1.552557425E-003 4.145258999E-003 3.525845181E-003 1.715448360E+001 -1.124864212E-003 2.818163236E-003 2.249282038E-003 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