# TOP STRAND, S1 # Oligo ID: R777 # Sequence (5'to 3'): TTCTACCTATGTGAT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R780 # Sequence (5'to 3'): ATCACATAGGTAGAA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: P6 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.003 # [KCl] = 0.050 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 02-27-2003 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- 1.347135576E+001 8.139499625E-003 1.924987036E-002 1.613124788E-002 1.356571216E+001 7.914577125E-003 1.852105414E-002 1.553585038E-002 1.366007292E+001 7.631897750E-003 1.984021232E-002 1.676698725E-002 1.375443804E+001 9.070498375E-003 1.949654559E-002 1.617265663E-002 1.384880751E+001 7.054991375E-003 1.995894493E-002 1.626320112E-002 1.394318134E+001 4.998614500E-003 1.966687003E-002 1.597419025E-002 1.403755953E+001 6.340665625E-003 2.087577971E-002 1.716108937E-002 1.413194207E+001 6.074245625E-003 2.081635854E-002 1.690961913E-002 1.422632897E+001 5.701897750E-003 2.060764807E-002 1.684909275E-002 1.432072023E+001 6.812714875E-003 1.990741105E-002 1.614722134E-002 1.441511584E+001 4.631535625E-003 1.921874388E-002 1.581378513E-002 1.450951581E+001 4.452938875E-003 1.862841851E-002 1.501522712E-002 1.460392013E+001 4.089177125E-003 1.921230857E-002 1.532656612E-002 1.469832881E+001 4.720446750E-003 1.917336040E-002 1.550994225E-002 1.479274185E+001 3.153261125E-003 1.859122097E-002 1.510159362E-002 1.488715924E+001 2.798670000E-003 1.793009890E-002 1.445996000E-002 1.498158099E+001 3.941486625E-003 1.593813903E-002 1.254547138E-002 1.507600710E+001 4.962395625E-003 1.631465696E-002 1.288485888E-002 1.517043756E+001 4.797090750E-003 1.648304660E-002 1.281230269E-002 1.526487238E+001 5.155810875E-003 1.498526484E-002 1.194891438E-002 1.535931156E+001 3.560421125E-003 1.411999804E-002 1.070934194E-002 1.545375509E+001 4.236138500E-003 1.535898673E-002 1.164648825E-002 1.554820298E+001 4.871889500E-003 1.494589068E-002 1.178963150E-002 1.564265522E+001 5.224532625E-003 1.468959881E-002 1.132827119E-002 1.573711182E+001 4.603542162E-003 1.337640096E-002 1.022411413E-002 1.583157278E+001 4.886044837E-003 1.360047275E-002 1.062815258E-002 1.592603809E+001 3.579258488E-003 1.260907928E-002 9.906238256E-003 1.602050776E+001 3.720749687E-003 1.199464656E-002 9.288130156E-003 1.611498179E+001 4.077363875E-003 1.161758724E-002 8.901893062E-003 1.620946017E+001 3.919325125E-003 1.148131963E-002 8.667682437E-003 1.630394291E+001 3.630749500E-003 1.147865177E-002 8.957925250E-003 1.639843000E+001 2.092067113E-003 1.151607907E-002 9.085066444E-003 1.649292146E+001 8.705141375E-004 1.251247320E-002 9.694095431E-003 1.658741726E+001 2.227820275E-003 1.171206109E-002 9.132587544E-003 1.668191743E+001 2.991072625E-003 1.173752024E-002 9.101421188E-003 1.677642195E+001 2.447954000E-003 1.262604096E-002 9.781532500E-003 1.687093083E+001 3.295102500E-004 1.177462865E-002 9.499894500E-003 1.696544406E+001 1.348602338E-003 1.264492730E-002 9.924123831E-003 1.705996165E+001 1.024383250E-003 1.024563224E-002 7.986764313E-003 1.715448360E+001 1.869589811E-003 1.032228276E-002 8.047045094E-003 1.724900990E+001 3.037441363E-003 8.834884545E-003 6.606429319E-003 1.734354056E+001 3.293379375E-003 9.298445515E-003 6.860483719E-003 1.743807557E+001 2.818527641E-003 9.042960740E-003 6.819501179E-003 1.753261494E+001 1.931951700E-003 8.927937221E-003 6.782869150E-003 1.762715867E+001 4.068617936E-003 9.306785592E-003 7.214361032E-003 1.772170676E+001 3.332457875E-003 9.205956713E-003 7.226071844E-003 1.781625920E+001 2.953663262E-003 7.614390009E-003 5.841240053E-003 1.791081599E+001 2.973628513E-003 8.430425455E-003 6.282275744E-003 1.800537715E+001 1.884966499E-003 9.239320927E-003 7.339764251E-003 1.809994266E+001 1.935045375E-003 1.035602809E-002 8.284704812E-003 1.819451252E+001 2.213783663E-003 9.795773741E-003 7.778096753E-003 1.828908675E+001 2.678195850E-003 9.837226605E-003 7.803822075E-003 1.838366533E+001 3.706936500E-003 1.052779134E-002 8.371841250E-003 1.847824826E+001 2.547230600E-003 1.133278038E-002 8.783952200E-003 1.857283555E+001 2.343744388E-003 1.089524697E-002 8.543532806E-003 1.866742720E+001 4.271223406E-003 9.864537680E-003 7.686868297E-003 1.876202321E+001 3.953898000E-003 1.101547488E-002 8.450201750E-003 1.885662357E+001 3.001657525E-003 1.276862404E-002 9.979131237E-003 1.895122828E+001 4.114401165E-003 1.198196897E-002 9.199348876E-003 1.904583736E+001 4.808701625E-003 1.002534196E-002 7.740185781E-003 1.914045079E+001 2.979484025E-003 9.987369160E-003 7.587070231E-003 1.923506857E+001 5.401331613E-003 9.092242220E-003 6.877498291E-003 1.932969072E+001 4.509302213E-003 8.646116788E-003 6.633348591E-003 1.942431721E+001 3.555595000E-003 8.765190685E-003 6.507354250E-003 1.951894807E+001 3.880064625E-003 9.120075286E-003 6.693030031E-003 1.961358328E+001 2.974015662E-003 1.016661475E-002 8.385762587E-003 1.970822285E+001 3.153269400E-003 1.092412428E-002 8.743211950E-003 1.980286677E+001 3.882202500E-003 8.647079512E-003 7.060870500E-003 1.989751505E+001 4.316507975E-003 8.426913149E-003 6.774094269E-003 1.999216769E+001 5.848413913E-003 9.071756440E-003 7.249213566E-003 2.008682469E+001 5.211414125E-003 8.449711118E-003 6.639664406E-003 2.018148604E+001 3.183351012E-003 7.156464190E-003 5.613154241E-003 2.027615174E+001 4.322755250E-003 8.168125750E-003 6.359850312E-003 2.037082180E+001 2.227097638E-003 7.142033670E-003 5.227502681E-003 2.046549622E+001 2.149369000E-003 8.429152552E-003 6.574997500E-003 2.056017500E+001 2.823774388E-003 7.485195314E-003 5.906151709E-003 2.065485813E+001 1.977115313E-003 6.930069917E-003 5.279182766E-003 2.074954562E+001 6.056203250E-004 6.290127759E-003 4.658360206E-003 2.084423746E+001 2.236808100E-003 5.565332181E-003 4.325258450E-003 2.093893366E+001 2.311073775E-003 4.318454913E-003 3.456183669E-003 2.103363422E+001 2.148896232E-003 3.175571109E-003 2.537520518E-003 2.112833913E+001 2.101304325E-003 4.252389778E-003 3.452681425E-003 2.122304840E+001 1.787557587E-003 4.251105198E-003 3.256090559E-003 2.131776203E+001 -1.318579250E-004 5.162148235E-003 3.871237194E-003 2.141248001E+001 1.120706737E-003 4.699681312E-003 3.499033262E-003 2.150720235E+001 7.207948875E-004 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4.059902700E-003 2.640984875E-003 2.435089823E+001 -9.655617500E-004 4.734192841E-003 3.348020875E-003 2.444575562E+001 6.876515125E-004 5.118179748E-003 3.952529366E-003 2.454061736E+001 7.390351075E-004 4.799382829E-003 3.705478393E-003 2.463548346E+001 7.498457750E-004 4.600148943E-003 3.152770975E-003 2.473035391E+001 7.058611900E-004 4.798590948E-003 3.855147060E-003 2.482522873E+001 2.348855450E-003 3.562420086E-003 2.573513663E-003 2.492010789E+001 3.314672775E-003 5.429356924E-003 4.143565919E-003 2.501499142E+001 1.463505175E-003 4.631543708E-003 3.449713619E-003 2.510987930E+001 4.830518500E-004 5.459350678E-003 4.054540438E-003 2.520477154E+001 1.357440625E-003 3.668693780E-003 2.754437719E-003 2.529966813E+001 2.659744125E-003 3.729125119E-003 2.873892594E-003 2.539456908E+001 1.469740875E-003 4.307388083E-003 3.381493656E-003 2.548947439E+001 1.334414937E-003 3.386387998E-003 2.855782563E-003 2.558438405E+001 5.448146875E-004 3.169926425E-003 2.696441312E-003 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1.125370293E-002 8.906042375E-003 2.985982770E+001 6.885815300E-003 1.326739760E-002 1.044673735E-002 2.995493776E+001 8.371710125E-003 1.368538222E-002 1.067158744E-002 3.005005217E+001 8.706562225E-003 1.319293913E-002 1.028146408E-002 3.014517094E+001 9.746740287E-003 1.361846876E-002 1.069073486E-002 3.024029406E+001 9.025373488E-003 1.495487798E-002 1.184865826E-002 3.033542154E+001 1.169098171E-002 1.412392685E-002 1.097638914E-002 3.043055338E+001 1.032460108E-002 1.523379890E-002 1.208952446E-002 3.052568957E+001 1.259433179E-002 1.700702005E-002 1.327882616E-002 3.062083012E+001 1.264540169E-002 1.700236641E-002 1.315544166E-002 3.071597503E+001 1.189892307E-002 1.654146846E-002 1.290732096E-002 3.081112429E+001 1.284570664E-002 1.682548480E-002 1.329888252E-002 3.090627791E+001 1.351953999E-002 1.687112453E-002 1.328218501E-002 3.100143589E+001 1.175124375E-002 1.698624006E-002 1.307764969E-002 3.109659822E+001 1.319802691E-002 1.800648525E-002 1.400092482E-002 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