# TOP STRAND, S1 # Oligo ID: R777 # Sequence (5'to 3'): TTCTACCTATGTGAT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R780 # Sequence (5'to 3'): ATCACATAGGTAGAA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: P8 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.010 # [KCl] = 0.050 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 10-04-2002 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- 1.914045079E+001 -8.307775000E-003 2.623611543E-002 2.054840667E-002 1.923506857E+001 -7.113861167E-003 2.612800106E-002 2.016947883E-002 1.932969072E+001 -7.175628333E-003 2.601630975E-002 1.997785889E-002 1.942431721E+001 -6.971639000E-003 2.598405465E-002 2.007724933E-002 1.951894807E+001 -6.015857000E-003 2.659127471E-002 2.044652800E-002 1.961358328E+001 -8.178684167E-003 2.685268910E-002 1.957028883E-002 1.970822285E+001 -5.580252833E-003 2.293972194E-002 1.770972856E-002 1.980286677E+001 -5.104010500E-003 2.075540038E-002 1.513610017E-002 1.989751505E+001 -6.028178833E-003 2.055465304E-002 1.529274744E-002 1.999216769E+001 -7.964940000E-003 2.173262868E-002 1.631462000E-002 2.008682469E+001 -6.924460000E-003 2.137706878E-002 1.660238667E-002 2.018148604E+001 -7.710050333E-003 1.991053043E-002 1.611791300E-002 2.027615174E+001 -9.737733000E-003 1.858612049E-002 1.507183033E-002 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