# TOP STRAND, S1 # Oligo ID: R777 # Sequence (5'to 3'): TTCTACCTATGTGAT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R780 # Sequence (5'to 3'): ATCACATAGGTAGAA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: P9 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.020 # [KCl] = 0.050 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 04-24-2003 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- 1.620946017E+001 -1.351388000E-002 3.494753241E-002 2.975443333E-002 1.630394291E+001 -1.411256467E-002 3.670939265E-002 3.110342356E-002 1.639843000E+001 -1.398744600E-002 3.461888768E-002 2.917817933E-002 1.649292146E+001 -1.212129483E-002 3.517908045E-002 3.014661344E-002 1.658741726E+001 -1.502731500E-002 3.648435074E-002 3.132195667E-002 1.668191743E+001 -1.490385200E-002 3.651969800E-002 3.113661533E-002 1.677642195E+001 -1.440340467E-002 3.591011144E-002 3.053282356E-002 1.687093083E+001 -1.531095183E-002 3.502486735E-002 2.987417544E-002 1.696544406E+001 -1.623157033E-002 3.573769908E-002 3.054911311E-002 1.705996165E+001 -1.515318933E-002 3.549486029E-002 3.037798711E-002 1.715448360E+001 -1.603787617E-002 3.445684976E-002 2.943517589E-002 1.724900990E+001 -1.391716833E-002 3.355925069E-002 2.866311778E-002 1.734354056E+001 -1.322225150E-002 3.176573204E-002 2.705484233E-002 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