# TOP STRAND, S1 # Oligo ID: R777 # Sequence (5'to 3'): TTCTACCTATGTGAT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R780 # Sequence (5'to 3'): ATCACATAGGTAGAA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: PN # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.000 # [KCl] = 0.050 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 12-06-2001 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1240000000E+02 -.3507734875E-03 .3362016605E-02 .2750717884E-02 .1250000000E+02 .4431790000E-03 .3313765960E-02 .2877117750E-02 .1260000000E+02 -.1015148375E-03 .2933851924E-02 .1979784419E-02 .1270000000E+02 .1574274038E-02 .3502056271E-02 .2880893213E-02 .1280000000E+02 -.4825425125E-03 .2642247027E-02 .1913321487E-02 .1290000000E+02 .4142755125E-03 .2652214444E-02 .2017903109E-02 .1300000000E+02 -.1173440700E-02 .2709203737E-02 .2098433550E-02 .1310000000E+02 .9423254125E-03 .3142826212E-02 .2406810941E-02 .1320000000E+02 -.5135100675E-03 .2766728145E-02 .1939071716E-02 .1330000000E+02 .2382008037E-02 .3585385755E-02 .2612872972E-02 .1340000000E+02 -.1995091939E-02 .3380043472E-02 .2454809296E-02 .1350000000E+02 -.1115013875E-02 .2617827255E-02 .1975294656E-02 .1360000000E+02 -.1582367717E-02 .2989100325E-02 .2375669032E-02 .1370000000E+02 -.1698759950E-02 .4668434155E-02 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