# TOP STRAND, S1 # Oligo ID: R777 # Sequence (5'to 3'): TTCTACCTATGTGAT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R780 # Sequence (5'to 3'): ATCACATAGGTAGAA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: Q6 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.0015 # [KCl] = 0.600 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 09-08-2003 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1610000000E+02 .8962579250E-03 .2008798379E-02 .1470717462E-02 .1620000000E+02 .1755893150E-02 .2042683742E-02 .1550195350E-02 .1630000000E+02 .4619724425E-03 .2275617567E-02 .1686148033E-02 .1640000000E+02 .7681717750E-03 .1801229346E-02 .1240487294E-02 .1650000000E+02 .1322122975E-02 .2792325802E-02 .2049151525E-02 .1660000000E+02 .1136158275E-02 .1276235734E-02 .9500954750E-03 .1670000000E+02 .2284693262E-03 .2254886476E-02 .1843320505E-02 .1680000000E+02 .7623578625E-03 .1749533824E-02 .1386656897E-02 .1690000000E+02 .7761778500E-03 .1817624245E-02 .1299205550E-02 .1700000000E+02 .1049957263E-02 .2016104719E-02 .1451163553E-02 .1710000000E+02 -.5202898500E-03 .1843665905E-02 .1479409400E-02 .1720000000E+02 -.5948612500E-05 .1950902853E-02 .1734172137E-02 .1730000000E+02 -.2908356625E-03 .2006606126E-02 .1666613497E-02 .1740000000E+02 -.4231516250E-04 .1806234736E-02 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