# TOP STRAND, S1 # Oligo ID: R777 # Sequence (5'to 3'): TTCTACCTATGTGAT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R780 # Sequence (5'to 3'): ATCACATAGGTAGAA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: Q7 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.0015 # [KCl] = 1.000 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 09-09-2003 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1620000000E+02 .6820072500E-02 .8444988126E-02 .5854687000E-02 .1630000000E+02 .8334148250E-02 .9338552602E-02 .6936951750E-02 .1640000000E+02 .6396716500E-02 .8197442239E-02 .5730401750E-02 .1650000000E+02 .6182240300E-02 .7327370020E-02 .5204999850E-02 .1660000000E+02 .6722102750E-02 .7422083427E-02 .5305177750E-02 .1670000000E+02 .5790718000E-02 .7018950526E-02 .4973991000E-02 .1680000000E+02 .4945947875E-02 .9331847407E-02 .6968431625E-02 .1690000000E+02 .4070257750E-02 .7883614762E-02 .5893856750E-02 .1700000000E+02 .4520455750E-02 .6870681563E-02 .4490321750E-02 .1710000000E+02 .4214529250E-02 .7326202526E-02 .5856204750E-02 .1720000000E+02 .5203580000E-02 .5518519271E-02 .3962750500E-02 .1730000000E+02 .4571270000E-02 .7098681972E-02 .5083544500E-02 .1740000000E+02 .4590885175E-02 .7944824199E-02 .6018801825E-02 .1750000000E+02 .3463177950E-02 .6871153086E-02 .5491415550E-02 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