# TOP STRAND, S1 # Oligo ID: R777 # Sequence (5'to 3'): TTCTACCTATGTGAT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R780 # Sequence (5'to 3'): ATCACATAGGTAGAA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: R4 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.010 # [KCl] = 0.100 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 08-19-2003 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1930000000E+02 .1719160000E-02 .4642741197E-02 .3617999250E-02 .1940000000E+02 .1297049250E-02 .7507313400E-02 .5683734500E-02 .1950000000E+02 -.1866179375E-03 .5874750540E-02 .4665317587E-02 .1960000000E+02 .1353810075E-02 .3902351045E-02 .3075312444E-02 .1970000000E+02 .8425660000E-03 .6251551866E-02 .5123133500E-02 .1980000000E+02 .1647932625E-02 .6900658165E-02 .5634099875E-02 .1990000000E+02 .1825854412E-02 .3648350197E-02 .2596296484E-02 .2000000000E+02 .2076236850E-02 .3913015189E-02 .3157673400E-02 .2010000000E+02 .1407908575E-02 .5942045619E-02 .4653070281E-02 .2020000000E+02 .1192231300E-02 .5263347878E-02 .3887101200E-02 .2030000000E+02 .1060080125E-02 .3063330162E-02 .2596346094E-02 .2040000000E+02 -.1031968750E-04 .5537727926E-02 .4278666266E-02 .2050000000E+02 .2052720100E-02 .3624864369E-02 .2731342125E-02 .2060000000E+02 .1920540400E-02 .3380710645E-02 .2943771350E-02 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