# TOP STRAND, S1 # Oligo ID: R777 # Sequence (5'to 3'): TTCTACCTATGTGAT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R780 # Sequence (5'to 3'): ATCACATAGGTAGAA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: R5 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.010 # [KCl] = 0.200 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 08-22-2003 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1920000000E+02 .2875511475E-02 .1086641807E-01 .8697753525E-02 .1930000000E+02 .3128688775E-02 .3683911761E-02 .3180650725E-02 .1940000000E+02 .5064671600E-02 .4090476455E-02 .2895580900E-02 .1950000000E+02 .6268203250E-02 .7107634650E-02 .6046931750E-02 .1960000000E+02 .8188539000E-02 .3934109160E-02 .3076707500E-02 .1970000000E+02 .4393897250E-02 .7310616146E-02 .5013391375E-02 .1980000000E+02 .3617228750E-02 .7058856035E-02 .5157491750E-02 .1990000000E+02 .9201800750E-02 .4423510982E-02 .3203938875E-02 .2000000000E+02 .2634025645E-02 .4422361806E-02 .3168067855E-02 .2010000000E+02 .1147099250E-02 .8171823055E-02 .6593268750E-02 .2020000000E+02 .3902456480E-02 .2930839352E-02 .1916817280E-02 .2030000000E+02 .6764704250E-02 .3738237791E-02 .2675573625E-02 .2040000000E+02 .3225130500E-02 .1510471064E-02 .1070731250E-02 .2050000000E+02 .6450358000E-02 .4367670153E-02 .3518176500E-02 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