# TOP STRAND, S1 # Oligo ID: R777 # Sequence (5'to 3'): TTCTACCTATGTGAT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R780 # Sequence (5'to 3'): ATCACATAGGTAGAA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: R6 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.010 # [KCl] = 0.600 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 08-28-2003 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1810000000E+02 .3861939700E-02 .6460057040E-02 .4443832550E-02 .1820000000E+02 .3707490625E-02 .5926830304E-02 .4273424125E-02 .1830000000E+02 .3628708125E-02 .5059693619E-02 .3695544125E-02 .1840000000E+02 .2131536062E-02 .5115154538E-02 .3462830938E-02 .1850000000E+02 .1921255275E-02 .4472186533E-02 .3259434656E-02 .1860000000E+02 .3467154250E-02 .6186488211E-02 .4476556562E-02 .1870000000E+02 .1915510437E-02 .5436664595E-02 .3833124922E-02 .1880000000E+02 .2884032850E-02 .4515255195E-02 .3880376363E-02 .1890000000E+02 .1449357800E-02 .5159037471E-02 .3912010700E-02 .1900000000E+02 .1854307275E-02 .5527982067E-02 .4354203156E-02 .1910000000E+02 .3393829959E-02 .4685292696E-02 .3553285791E-02 .1920000000E+02 .1865156863E-02 .3954043297E-02 .2968515387E-02 .1930000000E+02 .2295203625E-02 .5354133840E-02 .3914985219E-02 .1940000000E+02 .1612217475E-02 .4465669908E-02 .3135556894E-02 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