# TOP STRAND, S1 # Oligo ID: R777 # Sequence (5'to 3'): TTCTACCTATGTGAT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R780 # Sequence (5'to 3'): ATCACATAGGTAGAA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: R7 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.010 # [KCl] = 1.000 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 09-04-2003 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1990000000E+02 .1858203750E-01 .1078495463E-02 .7965462500E-03 .2000000000E+02 .1962064000E-01 .2179069454E-02 .1546910000E-02 .2010000000E+02 .1769889000E-01 .2833467641E-02 .2091745000E-02 .2020000000E+02 .1748141000E-01 .3501197533E-02 .2512995000E-02 .2030000000E+02 .1797793250E-01 .4806859905E-02 .3550898750E-02 .2040000000E+02 .1874572000E-01 .3740676166E-02 .2820050000E-02 .2050000000E+02 .1734762500E-01 .5034486971E-02 .3768612500E-02 .2060000000E+02 .1652667250E-01 .3902918754E-02 .2891043750E-02 .2070000000E+02 .1577299750E-01 .1476665466E-02 .1157442500E-02 .2080000000E+02 .1600723000E-01 .1707075749E-02 .1297380000E-02 .2090000000E+02 .1683232500E-01 .2193676958E-02 .1509052500E-02 .2100000000E+02 .1763336250E-01 .4127388973E-02 .3037421250E-02 .2110000000E+02 .1388161000E-01 .4325949859E-02 .3138455000E-02 .2120000000E+02 .1465639000E-01 .3792741651E-02 .2876395000E-02 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