# TOP STRAND, S1 # Oligo ID: R777 # Sequence (5'to 3'): TTCTACCTATGTGAT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R780 # Sequence (5'to 3'): ATCACATAGGTAGAA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: V2 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.050 # [KCl] = 0.050 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 09-30-2003 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1490000000E+02 .2858535865E-02 .5799632499E-02 .4006008453E-02 .1500000000E+02 .3559041125E-02 .5429864308E-02 .3587337344E-02 .1510000000E+02 .3739829750E-02 .4501774530E-02 .2763709875E-02 .1520000000E+02 .3077521950E-02 .5480096485E-02 .3943945312E-02 .1530000000E+02 .2836888750E-02 .5425979387E-02 .3757514062E-02 .1540000000E+02 .4485519800E-02 .5503312610E-02 .4238735500E-02 .1550000000E+02 .3463865375E-02 .4717971960E-02 .3235365031E-02 .1560000000E+02 .1995039250E-02 .4403405877E-02 .3133135438E-02 .1570000000E+02 .2387135500E-02 .3820925070E-02 .2646322750E-02 .1580000000E+02 .2858922625E-02 .4250171734E-02 .2535366062E-02 .1590000000E+02 .5726423875E-03 .4637125253E-02 .3738152362E-02 .1600000000E+02 .2201867750E-02 .3833797364E-02 .2575442687E-02 .1610000000E+02 .3157276875E-02 .3825544150E-02 .2804532344E-02 .1620000000E+02 .1471567250E-02 .3471270862E-02 .2602788937E-02 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