# TOP STRAND, S1 # Oligo ID: R777 # Sequence (5'to 3'): TTCTACCTATGTGAT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R780 # Sequence (5'to 3'): ATCACATAGGTAGAA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: V5 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.050 # [KCl] = 0.200 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 01-31-2005 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1740000000E+02 -.5108820950E-02 .1614290053E-01 .1433245655E-01 .1750000000E+02 -.1753135625E-02 .1502291039E-01 .1206817078E-01 .1760000000E+02 -.3647958750E-03 .1506738891E-01 .1238225588E-01 .1770000000E+02 -.2200354125E-02 .1507121718E-01 .1218213191E-01 .1780000000E+02 -.3181713325E-02 .1455901570E-01 .1227779501E-01 .1790000000E+02 .7833621250E-03 .1436292859E-01 .1217017659E-01 .1800000000E+02 .1199821250E-03 .1294051406E-01 .1054684509E-01 .1810000000E+02 -.1205961900E-02 .1016729831E-01 .7862335600E-02 .1820000000E+02 .2632610875E-02 .1043869988E-01 .9034102406E-02 .1830000000E+02 -.1312796250E-03 .9441338727E-02 .8113963781E-02 .1840000000E+02 .3910915500E-02 .1249290893E-01 .1059974163E-01 .1850000000E+02 .1827819000E-02 .8975693949E-02 .7880304000E-02 .1860000000E+02 -.2311802250E-03 .6603653347E-02 .5452631581E-02 .1870000000E+02 .1295258875E-02 .1055389261E-01 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