# TOP STRAND, S1 # Oligo ID: R777 # Sequence (5'to 3'): TTCTACCTATGTGAT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R780 # Sequence (5'to 3'): ATCACATAGGTAGAA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: W4 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.000 # [KCl] = 0.100 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 10-11-2003 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1730000000E+02 .1000629250E-01 .8044138811E-02 .5176358125E-02 .1740000000E+02 .9161074750E-02 .8829107512E-02 .5698142625E-02 .1750000000E+02 .7438567375E-02 .9742323306E-02 .6458166312E-02 .1760000000E+02 .1039271899E-01 .9787404204E-02 .5977380506E-02 .1770000000E+02 .1001790913E-01 .5650633322E-02 .4040460875E-02 .1780000000E+02 .8148084512E-02 .8633760074E-02 .5130635244E-02 .1790000000E+02 .8479462000E-02 .9407444323E-02 .6229251500E-02 .1800000000E+02 .8996712137E-02 .8793790080E-02 .5801449147E-02 .1810000000E+02 .8298363312E-02 .7882075818E-02 .5280213344E-02 .1820000000E+02 .5612343500E-02 .9676239318E-02 .6026405000E-02 .1830000000E+02 .6430953963E-02 .6445979394E-02 .5054298778E-02 .1840000000E+02 .3819346988E-02 .9235738362E-02 .5908215509E-02 .1850000000E+02 .5744143875E-02 .8967544998E-02 .6489998125E-02 .1860000000E+02 .2494861575E-02 .8931017455E-02 .6201316675E-02 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