# TOP STRAND, S1 # Oligo ID: R777 # Sequence (5'to 3'): TTCTACCTATGTGAT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R780 # Sequence (5'to 3'): ATCACATAGGTAGAA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: W5 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.000 # [KCl] = 0.200 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 10-15-2003 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1620000000E+02 .9624639250E-02 .5772061097E-02 .4416160750E-02 .1630000000E+02 .1182757475E-01 .6240790370E-02 .4319812625E-02 .1640000000E+02 .1223503125E-01 .8145966782E-02 .5836834375E-02 .1650000000E+02 .1261322750E-01 .5763935841E-02 .4222226250E-02 .1660000000E+02 .1519443825E-01 .8377604410E-02 .6539016750E-02 .1670000000E+02 .1219526475E-01 .7491813209E-02 .5440737625E-02 .1680000000E+02 .1074342700E-01 .7137143545E-02 .5289946500E-02 .1690000000E+02 .1063661000E-01 .8470601064E-02 .6004235000E-02 .1700000000E+02 .9917923500E-02 .9707714635E-02 .7149298250E-02 .1710000000E+02 .1113167900E-01 .5575519019E-02 .3968590500E-02 .1720000000E+02 .7956159750E-02 .5212364405E-02 .3398019750E-02 .1730000000E+02 .1261075650E-01 .3497198074E-02 .2553880250E-02 .1740000000E+02 .7082234500E-02 .2502832670E-02 .1957042500E-02 .1750000000E+02 .7499299000E-02 .4903706015E-02 .3435155500E-02 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