# TOP STRAND, S1 # Oligo ID: R777 # Sequence (5'to 3'): TTCTACCTATGTGAT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R780 # Sequence (5'to 3'): ATCACATAGGTAGAA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: W7 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.000 # [KCl] = 1.000 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 09-22-2003 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1590000000E+02 .4219057738E-02 .5045735295E-02 .3613615144E-02 .1600000000E+02 .5538163125E-02 .4049717279E-02 .3076988344E-02 .1610000000E+02 .4071290412E-02 .4397644296E-02 .3316901881E-02 .1620000000E+02 .3730188425E-02 .5277969686E-02 .3477210112E-02 .1630000000E+02 .4280156928E-02 .3384826324E-02 .2850511322E-02 .1640000000E+02 .3688734663E-02 .4048003186E-02 .2995570253E-02 .1650000000E+02 .4133492500E-02 .4819222983E-02 .3557884875E-02 .1660000000E+02 .2208871063E-02 .4802137772E-02 .3743289422E-02 .1670000000E+02 .3281052450E-02 .3952533855E-02 .3110733938E-02 .1680000000E+02 .2944231750E-02 .3808566644E-02 .2942151812E-02 .1690000000E+02 .3726505625E-02 .5150822688E-02 .3725747781E-02 .1700000000E+02 .4022351250E-02 .4096527284E-02 .3075391687E-02 .1710000000E+02 .2259870650E-02 .4001236688E-02 .2736603187E-02 .1720000000E+02 .4337010250E-02 .3217752007E-02 .1969087187E-02 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