# TOP STRAND, S1 # Oligo ID: R777 # Sequence (5'to 3'): TTCTACCTATGTGAT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R780 # Sequence (5'to 3'): ATCACATAGGTAGAA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: Y4 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.003 # [KCl] = 0.100 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 10-24-2003 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1620000000E+02 .6235392625E-02 .3263066149E-02 .2567875781E-02 .1630000000E+02 .3594916625E-02 .2821090281E-02 .2354815531E-02 .1640000000E+02 .5569151350E-02 .3770549607E-02 .2829635725E-02 .1650000000E+02 .3305933888E-02 .3783961945E-02 .3065548112E-02 .1660000000E+02 .3744022550E-02 .5096531243E-02 .3101888337E-02 .1670000000E+02 .4568469375E-02 .4497908019E-02 .3547785719E-02 .1680000000E+02 .1753994625E-02 .4371805178E-02 .3134838063E-02 .1690000000E+02 .4738533750E-02 .3088592592E-02 .2019776187E-02 .1700000000E+02 .3840690762E-02 .2435551783E-02 .1621315988E-02 .1710000000E+02 .2939658250E-02 .3240280931E-02 .2536984750E-02 .1720000000E+02 .2787903837E-02 .3281132290E-02 .2431671122E-02 .1730000000E+02 .4474061400E-02 .3202997449E-02 .2852656600E-02 .1740000000E+02 .2342195237E-02 .1884599790E-02 .1493257013E-02 .1750000000E+02 .1986565600E-02 .4104515085E-02 .3428688900E-02 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