# TOP STRAND, S1 # Oligo ID: R777 # Sequence (5'to 3'): TTCTACCTATGTGAT # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R780 # Sequence (5'to 3'): ATCACATAGGTAGAA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: Z4 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.020 # [KCl] = 0.100 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 10-23-2003 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1320000000E+02 .1377049950E-01 .6352576754E-02 .5431170500E-02 .1330000000E+02 .1271203475E-01 .9043309610E-02 .7811515250E-02 .1340000000E+02 .1303004300E-01 .5109297350E-02 .3368942000E-02 .1350000000E+02 .1377405550E-01 .4716542685E-02 .3996154500E-02 .1360000000E+02 .1066721450E-01 .4857983405E-02 .4047260500E-02 .1370000000E+02 .1015283325E-01 .4845203224E-02 .3981191750E-02 .1380000000E+02 .1043307125E-01 .7146948098E-02 .5813903750E-02 .1390000000E+02 .1067650025E-01 .8577856912E-02 .6797479750E-02 .1400000000E+02 .1344499700E-01 .7959261572E-02 .6478068000E-02 .1410000000E+02 .1337927750E-01 .4038430977E-02 .2656716250E-02 .1420000000E+02 .1034367825E-01 .2871224049E-02 .2418716750E-02 .1430000000E+02 .1109184725E-01 .6482364771E-02 .4984347750E-02 .1440000000E+02 .9528189250E-02 .6454778989E-02 .4995430750E-02 .1450000000E+02 .8299522000E-02 .7092157699E-02 .5759653000E-02 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