# TOP STRAND, S1 # Oligo ID: R778 # Sequence (5'to 3'): GCAGTGGATGTGAGA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R781 # Sequence (5'to 3'): TCTCACATCCACTGC # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: D6 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.125 # [KCl] = 0.600 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 12-01-2003 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1670000000E+02 .1231476201E-01 .2530339322E-01 .1823613349E-01 .1680000000E+02 .1356088987E-01 .2601825044E-01 .1790107506E-01 .1690000000E+02 .1038374837E-01 .2750215032E-01 .1845215122E-01 .1700000000E+02 .1406541487E-01 .2677630896E-01 .1769114134E-01 .1710000000E+02 .1456269800E-01 .2769701019E-01 .1870680650E-01 .1720000000E+02 .1191591938E-01 .2167685082E-01 .1617169297E-01 .1730000000E+02 .1223650962E-01 .2507887699E-01 .1822198528E-01 .1740000000E+02 .1235364388E-01 .2394304785E-01 .1761452459E-01 .1750000000E+02 .1293278842E-01 .2448787911E-01 .1772535368E-01 .1760000000E+02 .1201804287E-01 .2287851078E-01 .1689119534E-01 .1770000000E+02 .1236193688E-01 .2682723796E-01 .1882085984E-01 .1780000000E+02 .8383448625E-02 .2201844906E-01 .1637851103E-01 .1790000000E+02 .1226399187E-01 .2727199132E-01 .1921754406E-01 .1800000000E+02 .9265651500E-02 .2180029083E-01 .1529204538E-01 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