# TOP STRAND, S1 # Oligo ID: R778 # Sequence (5'to 3'): GCAGTGGATGTGAGA # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BOTTOM STRAND, S2 # Oligo ID: R781 # Sequence (5'to 3'): TCTCACATCCACTGC # Concentration (mol/liter): 1.00e-06 # --------------------------------------------------------------------------- # BUFFER # Buffer ID: D7 # pH = 8.3 # Composition (mol/liter) # [MgCl2] = 0.125 # [KCl] = 1.000 # [Tris] = 0.010 # --------------------------------------------------------------------------- # Absorbance was collected at 268 nm and converted to # fractions of melted base pairs. # Heating and cooling melting profiles were averaged. # Date of experiment: 12-06-2003 # --------------------------------------------------------------------------- # # # # # | |Standard deviation | Average error # Temperature | Average fraction of | of fraction of | of fraction of # (Celsius) | melted base pairs | melted base pairs | melted base pairs # --------------------------------------------------------------------------------- .1610000000E+02 -.5367471000E-03 .5929465860E-02 .4924182150E-02 .1620000000E+02 -.1131903500E-02 .5181350971E-02 .4329286875E-02 .1630000000E+02 .2247184500E-02 .4341542303E-02 .3308114625E-02 .1640000000E+02 .8732802500E-03 .3987976690E-02 .3052328688E-02 .1650000000E+02 .5873972500E-03 .5901275184E-02 .4389177250E-02 .1660000000E+02 -.1109617750E-02 .3917893742E-02 .3481825562E-02 .1670000000E+02 -.5344685375E-03 .4949335673E-02 .3723238847E-02 .1680000000E+02 -.8901406250E-04 .6628682845E-02 .4932798031E-02 .1690000000E+02 .4803997500E-03 .5552526078E-02 .4676190313E-02 .1700000000E+02 .1298805988E-02 .3742006190E-02 .2970600266E-02 .1710000000E+02 .3552024625E-03 .5367726945E-02 .4113662038E-02 .1720000000E+02 -.1113447500E-03 .5674315777E-02 .4568757063E-02 .1730000000E+02 .9892657000E-03 .5979565154E-02 .4571802550E-02 .1740000000E+02 .2104932375E-02 .6555643252E-02 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